malonaldehyde dabsylpyrazole

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 偶氮苯
• 英文名称: malonaldehyde dabsylpyrazole
• 英文别名: N,N-dimethyl-4-[(4-pyrazol-1-ylsulfonylphenyl)diazenyl]aniline
• 化学式: C₁₇H₁₇N₅O₂S
• 分子量: 355.42
• InChiKey: CFBKJWPWVGRQJP-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.4
• 重原子数：
  25
• 可旋转键数：
  5
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.12
• 拓扑面积：
  88.3
• 氢给体数：
  0
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  1,1,3,3-四甲氧基丙烷 、 丹磺酰肼 在 盐酸 作用下， 以 二氯甲烷 为溶剂， 反应 1.0h， 生成 malonaldehyde dabsylpyrazole
  参考文献：
  名称：

  Determination of Aldehydic Lipid Peroxidation Products With Dabsylhydrazine by High-Performance Liquid Chromatography

  摘要：

  A high-performance liquid chromatographic method has been developed to measure adlehyde products of lipid peroxidation including n-alkanals, trans-2-alkenals, 4-hydroxy-2-nonenal, and malonaldehyde. The chromophoric reagent dabsylhydrazine reacts with various aldehydes produced from two different lipid peroxidation model systems: Fe2+/H2O2 and Fe3+/vitamin C oxidation of arachidonic and linoleic acids, respectively. The formed products were carefully characterized by IR, MS, and NMR spectral analysis and proved to be hydrazone rather than 2-pyrazoline or pyrrole derivatives. EI-MS of all the derivatized straight-chain aldehydes showed a characteristic (M - 28) fragment in the spectra with high consistency. Physicochemical properties including melting points are also described. The detection limits in the range of 11 pmol (5 ng) have been reached by using an NP Octadecyl C-18 reversed-phase column. 4-Hydroxy-2-nonenal and hexanal were found to be the most abundant aldehydes in the lipid peroxidation systems described. The dose-response curve showed great linearity and exhibited good reproducibility of this procedure for quantitative estimation.

  DOI：

  10.1021/ac00105a020

文献信息

• Determination of Aldehydic Lipid Peroxidation Products With Dabsylhydrazine by High-Performance Liquid Chromatography
  作者：Hung-Yi. Wu、Jen-Kun. Lin

  DOI：10.1021/ac00105a020

  日期：1995.5.1

  A high-performance liquid chromatographic method has been developed to measure adlehyde products of lipid peroxidation including n-alkanals, trans-2-alkenals, 4-hydroxy-2-nonenal, and malonaldehyde. The chromophoric reagent dabsylhydrazine reacts with various aldehydes produced from two different lipid peroxidation model systems: Fe2+/H2O2 and Fe3+/vitamin C oxidation of arachidonic and linoleic acids, respectively. The formed products were carefully characterized by IR, MS, and NMR spectral analysis and proved to be hydrazone rather than 2-pyrazoline or pyrrole derivatives. EI-MS of all the derivatized straight-chain aldehydes showed a characteristic (M - 28) fragment in the spectra with high consistency. Physicochemical properties including melting points are also described. The detection limits in the range of 11 pmol (5 ng) have been reached by using an NP Octadecyl C-18 reversed-phase column. 4-Hydroxy-2-nonenal and hexanal were found to be the most abundant aldehydes in the lipid peroxidation systems described. The dose-response curve showed great linearity and exhibited good reproducibility of this procedure for quantitative estimation.