TX synthase

• 分子结构分类: 转移酶 - 转移含磷基团 - 核苷酸转移酶
• 英文名称: TX synthase

反应信息

• 作为试剂：
  描述：

  [5,6,8,9,11,12,14,15-3H(N)]-花生四烯酸 在 4(3H)-喹唑啉酮,6-氨基-3-(3-氯-2-甲基苯基)-2-甲基- 、 TX synthase hemin 、 肾上腺素 作用下， 以 乙醇 为溶剂， 反应 0.58h， 以25%的产率得到12-hydroxy-[5,6,8,9,11,12-(3)H]-heptadecatrienoic acid
  参考文献：
  名称：

  Enzymatic synthesis of tritium labelled eicosanoids

  摘要：

  Methods for the synthesis of tritium labelled prostaglandins, thromboxanes, and hydroxy-derivatives of eicosapolyenoic acids using mono-, two-, and three-enzyme systems have been developed. Optimization of the eicosanoid syntheses with respect to the concentrations of enzymes and eicosapolyenoic acid. time, and temperature of incubation are reported. It is demonstrated that a number of compounds can be produced by enzymatic modifications of prostaglandins and thromboxane. Procedures for the preparation of twenty tritium labelled compounds are described.

  DOI：

  10.1002/(sici)1099-1344(1998080)41:8<741::aid-jlcr123>3.0.co;2-w