Fast Black K salt | 92-34-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 偶氮苯
• 英文名称: Fast Black K salt
• 英文别名: 2,5-Dimethoxy-4-[(E)-(4-nitrophenyl)diazenyl]benzene-1-diazonium chloride；2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]benzenediazonium;chloride
• CAS: 92-34-2
• 化学式: C₁₄H₁₂N₅O₄*Cl
• 分子量: 349.733
• InChiKey: NPLCKAVEFAKLMT-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  1.52
• 重原子数：
  24
• 可旋转键数：
  4
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  117
• 氢给体数：
  0
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  Fast Black K salt 在 吡啶 、 碳酸氢钠 作用下， 以 甲醇 、 水 为溶剂， 反应 73.33h， 生成 4-nitrobenzylazo-2',5'-dimethoxybenzylazo-4'''-N,N-(2-hydroxyethyl)-(2-O-(4,4'-dimethoxytrityl)ethyl)azobenzene
  参考文献：
  名称：

  Dark quenchers for donor-acceptor energy transfer

  摘要：

  本发明提供了一类名为黑洞猝灭剂（“BHQs”）的暗猝灭剂家族，它们是有效的激发态能量猝灭剂，但本身基本上不发光。还提供了使用BHQs的方法，包括将BHQs纳入探针以及使用探针的方法。

  公开号：

  US09139610B2
• 作为产物：
  描述：

  2,5-二甲氧基-4-[(4-硝基苯基)偶氮]苯胺 在 盐酸 、 sodium nitrite 作用下， 以 水 为溶剂， 反应 0.5h， 生成 Fast Black K salt
  参考文献：
  名称：

  [EN] ORGANIC SOLVENT SOLUBLE METAL COMPLEX AZO DYES
  [FR] COLORANTS AZOIQUES DE COMPLEXE METALLIQUE SOLUBLES DANS UN SOLVANT ORGANIQUE

  摘要：

  公开号：

  WO2005030875A3

文献信息

• Cell-penetrating poly(disulfide)-based nanoquenchers (<i>q</i>CPDs) for self-monitoring of intracellular gene delivery
  作者：Wenjie Lang、Si Si Liew、Shuyi Wang、Dawei Hong、Liquan Zhu、Shubo Du、Linye Jiang、Shao Q. Yao、Jingyan Ge

  DOI：10.1039/d1cc07020g

  日期：——

  Monitoring gene delivery has significant benefits in gene therapy. Herein, we report a nanoquencher system by doping a FRET pair during nucleic acid-assisted cell penetrating poly(disulfide) (CPD) formation. Our results show that this strategy not only produces an efficient gene delivery polymer with minimal endolysosomal trapping, but also enables monitoring the release of the gene from the vehicle

  监测基因传递对于基因治疗具有显着的益处。在此，我们报道了一种纳米猝灭剂系统，该系统通过在核酸辅助细胞穿透聚二硫醚（CPD）形成过程中掺杂 FRET 对来实现。我们的结果表明，该策略不仅产生具有最小内溶酶体捕获的有效基因递送聚合物，而且还能够监测活细胞中载体中基因的释放。这项研究进一步扩展了 CPD 作为基因传递工具的应用。
• Fluorescence Quenchers for Hydrazone and Oxime Orthogonal Bioconjugation
  作者：Pete Crisalli、Armando R. Hernández、Eric T. Kool

  DOI：10.1021/bc300344b

  日期：2012.9.19

  We describe the synthesis and properties of new fluorescence quenchers containing aldehyde, hydrazine, and aminooxy groups, allowing convenient bioconjugation as oximes or hydrazones. Conjugation to oligonucleotides proceeded in high yield with aniline as catalyst. Kinetics studies of conjugation show that, under optimal conditions, a hydrazine or aminooxy quencher can react with aldehyde-modified DNA to form a stable hydrazone or oxime adduct in as little as five minutes. The resulting quencher-containing DNAs were assessed for their ability to quench the emission of fluorescein in labeled complements and compared to the commercially available dabcyl and Black Hole Quencher 2 (BHQ2), which were conjugated as phosphoramidites. Results show that the new quenchers possess slightly different absorbance properties compared to dabcyl and are as efficient as the commercial quenchers in quenching fluorescein emission. Hydrazone-based quenchers were further successfully incorporated into molecular beacons and shown to give high signal to background ratios in single nucleotide polymorphism detection in vitro. Finally, aminooxy and hydrazine quenchers were applied to quenching of an aldehyde-containing fluorophore associated with living cells, demonstrating cellular quenching within one hour.