3-(2'-螺旋金刚烷)-4-甲氧基-4-(3"-磷酰氧基)苯-1,2-二氧杂环丁烷 | 122341-56-4

• 物质功能分类: 化学试剂 - 有机试剂 - 膦配体
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 中文名称: 3-(2'-螺旋金刚烷)-4-甲氧基-4-(3"-磷酰氧基)苯-1,2-二氧杂环丁烷
• 中文别名: 3-(2'-螺旋金刚烷)-4-甲氧基-4-(3''-磷酰氧基)苯-1,2-二氧杂环丁烷；3-(2-螺旋金刚烷)-4-甲氧基-4-(3-磷氧酰)-苯基-1,2-二氧环乙烷(AMPPD)；3-(2-螺旋金刚烷)-4-甲氧基-4-(3-磷氧酰)-苯基-1,2-二氧环乙烷；3-(2-螺旋金刚烷)-4-甲氧基-(3-磷氧酰)-苯基；3-(2-螺旋金刚烷)-4-甲氧基-(3-磷氧酰)-苯基-1,2二氧环乙烷
• 英文名称: Amppd
• 英文别名: [3-(3'-methoxyspiro[adamantane-2,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
• CAS: 122341-56-4
• 化学式: C₁₈H₂₃O₇P
• 分子量: 382.3
• InChiKey: XYIPYISRNJUPBA-UHFFFAOYSA-N

物化性质

• 沸点：
  548.6±60.0 °C(Predicted)
• 密度：
  1.47±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  1.7
• 重原子数：
  26
• 可旋转键数：
  4
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  94.4
• 氢给体数：
  2
• 氢受体数：
  7

制备方法与用途

化学发光底物

AMPPD 是一种化学发光试剂，与鲁米诺同属氧化还原型发光试剂，适用于磷酸酶的超高灵敏度检测试剂。

主要性质

在碱性条件下，AMPPD 被 AP 酶解生成相当稳定的 AMP-D 阴离子。其分解半衰期为 2～30 分钟，在 15 分钟时达到最大光强度，并在接下来的 15～60 分钟内保持相对稳定。发出波长为 470nm 的持续性光。

用途

AMPPD 是碱性磷酸酶的化学发光底物，适用于在适宜缓冲液中通过酶催化水解作用分解成 AMP-D 并产生高强度光信号。发光速度取决于碱磷酶浓度。当碱磷酶偶合到杂交探针时，可以通过此系统检测杂交分子的存在量。

优势

反应速度快，在很短时间内可提供正确可靠的结果。

应用原理

AMPPD 采用微粒子化学发光技术，利用最新磁性微粒包被抗体。用碱性磷酸酶（ALP）标记抗原（抗体）。经过普通抗原抗体反应后，碱性磷酸酶结合在微粒子上，并与待测物质的浓度成比例。通过洗涤步骤（反应管两边有磁场，磁性微粒包被的抗原抗体结合物被吸附在管子两端，其余游离部分被抽吸掉），最后加入发光底物 Dioxetane Phosphate，5 分钟后仪器通过光电倍增管检测反应的发光强度。

生物活性

AMPPD 是 1,2 - dioxo-cyclohexane 衍生物，具有超高的碱性磷酸酶底物活性。

文献信息

• Non-specific reaction inhibitor, method for inhibiting non-specific reaction, and kit
  申请人：Okamura Yoshikazu

  公开号：US11125742B2

  公开（公告）日：2021-09-21

  Provided is a non-specific reaction inhibitor for achieving the accurate detection and quantitation of a trace component (a target substance) contained in a sample, in an immunoassay, by simply and effectively inhibiting a non-specific reaction associated with the measurement. The non-specific reaction inhibitor comprises a substance of the formula I: wherein R1 and R2 together form a double bond between carbons, to which they are respectively bonded directly, or R1 is a hydrogen atom and R2 is a group formed by removing H from an SH-group-containing compound, B is a support, and L is a spacer arm portion.

  提供的是一种非特异性反应抑制剂，用于通过简单有效地抑制与测量相关的非特异性反应，在免疫测定中实现对样品中微量组分（目标物质）的准确检测和定量。该非特异性反应抑制剂包括式I的物质：其中R1和R2共同形成碳之间的双键，它们分别直接连接在碳上，或者R1是氢原子，R2是从含有SH基团的化合物中去除H形成的基团，B是支持体，L是空间臂部分。
• METHOD FOR IMMUNOLOGICALLY MEASURING SOLUBLE LR11
  申请人：Sekisui Medical Co., Ltd.

  公开号：EP2708893A1

  公开（公告）日：2014-03-19

  To provide a method for assaying soluble LR11 in a biological sample, which method realizes a simple and accurate assay of soluble LR11 present in the sample by immunological means without requiring isolation of soluble LR11 from the biological sample (e.g., a serum sample). The method of the invention for immunologically assaying soluble LR11 present in a biological sample, characterized in that the method includes treating the sample with at least one surfactant selected from among one or more sulfobetaine amphoteric surfactants and one or more amidosulfobetaine amphoteric surfactants.

  提供一种检测生物样品中可溶性LR11的方法，该方法通过免疫学手段实现对样品中可溶性LR11的简单而准确的检测，而不需要从生物样品（如血清样品）中分离出可溶性LR11。 本发明的免疫学检测生物样品中存在的可溶性 LR11 的方法，其特征在于该方法包括用至少一种选自一种或多种磺基甜菜碱两性表面活性剂和一种或多种脒基磺基甜菜碱两性表面活性剂的表面活性剂处理样品。
• In situ chemiluminescent substrates and assays
  申请人：Sparks Alison

  公开号：US10031142B2

  公开（公告）日：2018-07-24

  Methods for generating a chemiluminescent enzyme substrate in situ, in aqueous or other assay conditions. Also disclosed are methods to use the substrates to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Kits relating to these methods are also disclosed.

  在水溶液或其他检测条件下原位生成化学发光酶底物的方法。还公开了使用底物产生光、检测和/或量化酶、抗原和/或核酸的方法。还公开了与这些方法有关的试剂盒。
• Protease inhibitor assay
  申请人：——

  公开号：US20010031478A1

  公开（公告）日：2001-10-18

  Heterogenous and homogenous assays are provided for the detection of protease inhibitory activity in a sample or target compound, taking advantage of the chemiluminescent characteristics of 1,2-dioxetanes. In the heterogenous assay, a peptide bearing a cleavage site for the protease of interest is provided with a first member of a first ligand binding pair at one end, and a first member of a second ligand binding pair at the other end. The other member of the first ligand binding pair is attached to a surface, which binds the peptide, or protease substrate, to the surface. The peptide substrate is combined with the protease and target compound or sample. Substrate cleavage, if not inhibited, is allowed to occur, and any unbound cleaved fragments are removed. An enzyme complexed with the second member of the second ligand binding pair is added, and allowed to bind to any of the (uncleaved) first member of the second ligand binding pair remaining. Unbound complex is removed, and a 1,2-dioxetane substrate for the enzyme is added. If any peptide substrate has not been cleaved, the dioxetane will chemiluminesce, indicating inhibitory activity. In a homogenous assay, the same substrate bears at one end a fluorescent energy accepting moiety, and at the other end a 1,2-dioxetane or precursor. If the substrate is cleaved by the protease, the dioxetane and the fluorescent moiety are not in close physical relationship, and no energy transfer occurrs when the dioxetane is caused to decompose. If cleavage has not occurred, indicating inhibition, when the dioxetane is caused to decompose, energy is transferred to the fluorescing entity, which releases light of a wavelength recognizably distinct from that of the dioxetane.

  利用 1,2-二氧杂环丁烷的化学发光特性，提供了用于检测样品或目标化合物中蛋白酶抑制活性的异源和同源检测方法。在异源检测法中，带有相关蛋白酶裂解位点的多肽一端是第一配体结合对的第一成员，另一端是第二配体结合对的第一成员。第一配体结合对的另一端连接到表面上，从而将多肽或蛋白酶底物结合到表面上。多肽底物与蛋白酶和目标化合物或样品结合。如果底物裂解没有受到抑制，就会发生裂解，任何未结合的裂解片段都会被移除。加入与第二配体结合对的第二成员复合的酶，并允许其与第二配体结合对的第一成员（未裂解的）残留物结合。移除未结合的复合物，然后加入酶的 1,2- 二氧杂环丁烷底物。如果任何肽底物尚未被裂解，二氧杂环丁烷就会发生化学发光，表明其具有抑制活性。在同源检测中，同一种底物的一端是接受荧光能量的分子，另一端是 1,2-二氧杂环丁烷或前体。如果底物被蛋白酶裂解，二氧杂环丁烷和荧光分子之间的物理关系就不紧密，二氧杂环丁烷分解时就不会发生能量转移。如果二氧杂环丁烷没有发生裂解，表明二氧杂环丁烷受到抑制，当二氧杂环丁烷分解时，能量就会转移到荧光实体上，荧光实体就会释放出波长与二氧杂环丁烷波长明显不同的光。
• Cell growth, induction and lysis in an antibody-coated microplate for use in an elisa
  申请人：——

  公开号：US20030008325A1

  公开（公告）日：2003-01-09

  A competitive assay to determine the presence and concentration of an intracellular analyte (e.g., cAMP) in a sample is provided. All of the steps of the assay can be performed on the same assay plate, thereby eliminating the need to transfer the cells from a tissue culture plate on which the cells are grown, induced and lysed to a separate assay plate. The assay procedure includes combining, in a reaction chamber provided with a capture antibody, an antibody for the analyte, the sample to be assayed, and a conjugate of the analyte and an enzyme such as alkaline phosphatase. The mixture is incubated and washed and an enzyme labile substrate (e.g., a chemiluminescent, fluorescent or calorimetric substrate) is added. The assay can also be performed with a tagged analyte (e.g., an analyte having a radioactive or fluorescent tag) instead of an enzyme conjugate.

  本发明提供了一种竞争测定法，用于确定样品中细胞内分析物（如 cAMP）的存在和浓度。化验的所有步骤都可在同一化验板上进行，因此无需将细胞从其上生长、诱导和裂解的组织培养板转移到单独的化验板上。化验程序包括：在装有捕获抗体的反应室中，将分析物抗体、待化验样品以及分析物与碱性磷酸酶等酶的共轭物结合在一起。混合物经孵育和洗涤后加入酶标记底物（如化学发光、荧光或热量测定底物）。也可以用标记分析物（如具有放射性或荧光标记的分析物）代替酶结合物进行检测。