4-phenylazo-benzenesulfonic acid dimethylamide | 102840-88-0

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 偶氮苯
• 英文名称: 4-phenylazo-benzenesulfonic acid dimethylamide
• 英文别名: 4-Phenylazo-benzolsulfonsaeure-dimethylamid
• CAS: 102840-88-0
• 化学式: C₁₄H₁₅N₃O₂S
• 分子量: 289.358
• InChiKey: QKOSJMAKVUVMFQ-FOCLMDBBSA-N

文献信息

• [EN] REDUCTIVE RELEASE PROBES CONTAINING A CHEMOSELECTIVELY CLEAVABLE ALPHA-AZIDOETHER LINKER AND METHODS OF USE THEREOF<br/>[FR] SONDES À LIBÉRATION RÉDUCTRICE CONTENANT UN LIANT ALPHA-AZIDOÉTHER CLIVABLE DE FAÇON CHÉMIOSÉLECTIVE ET LEURS PROCÉDÉS D'UTILISATION
  申请人：UNIV LELAND STANFORD JUNIOR

  公开号：WO2011005821A1

  公开（公告）日：2011-01-13

  Probes comprising one or more selectively cleavable α-azidoether moieties are provided; and linkers comprising the one or more selectively cleavable α-azidoether moieties. The α-azidoether moiety will undergo a Staudinger reaction with a suitable reducing agent, resulting in cleavage. The probes find use in a variety of detection assays, e.g. specific polynucleotide binding assays, polypeptide binding assays, etc. The cleavable linkers are suitable for synthetic reactions, e.g. to prepare probes of the invention; in the synthesis of cleavable peptide conjugates; and the like.

  提供了包含一个或多个可选择裂解的α-叠氮醚基团的探针；以及包含一个或多个可选择裂解的α-叠氮醚基团的连接剂。α-叠氮醚基团将与适当的还原剂发生斯陶丁格反应，导致裂解。这些探针可用于各种检测分析中，例如特定多核苷酸结合分析、多肽结合分析等。可裂解的连接剂适用于合成反应，例如用于制备本发明的探针；用于合成可裂解的肽共轭物等。
• REDUCTIVE RELEASE PROBES CONTAINING A CHEMOSELECTIVELY CLEAVABLE ALPHA-AZIDOETHER LINKER AND METHODS OF USE THEREOF
  申请人：Franzini Raphael M.

  公开号：US20120178086A1

  公开（公告）日：2012-07-12

  Probes comprising one or more selectively cleavable α-azidoether moieties are provided; and linkers comprising the one or more selectively cleavable α-azidoether moieties. The α-azidoether moiety will undergo a Staudinger reaction with a suitable reducing agent, resulting in cleavage. The probes find use in a variety of detection assays, e.g. specific polynucleotide binding assays, polypeptide binding assays, etc. The cleavable linkers are suitable for synthetic reactions, e.g. to prepare probes of the invention; in the synthesis of cleavable peptide conjugates; and the like.

  提供了包含一个或多个可选择性裂解的α-叠氮醚基团的探针；以及包含一个或多个可选择性裂解的α-叠氮醚基团的连接体。α-叠氮醚基团将与合适的还原剂发生斯陶丁格反应，导致裂解。这些探针适用于各种检测分析中，例如特定的多核苷酸结合分析、多肽结合分析等。可裂解的连接体适用于合成反应，例如制备本发明的探针；在裂解肽共轭物的合成中使用等。
• NUCLEIC ACID PROBE, NUCLEIC ACID CHIP, METHOD OF DETECTING TARGET NUCLEIC ACID, METHOD OF SCREENING DRUG, APPARATUS FOR DETECTING TARGET NUCLEIC ACID AND GENE DIAGNOSIS METHOD
  申请人：Kyoto University

  公开号：EP1717313A1

  公开（公告）日：2006-11-02

  The nucleic acid probe of the invention is a nucleic acid probe for detecting a trace amount of target nucleic acid highly sensitively, highly accurately, and highly quickly, and is designed so that the nucleic acid probe undergoes a conformational change after hybridization to the target nucleic acid, the nucleic acid probe, which underwent the conformational change, has a decreased binding strength of hybridization and dissociates from the target nucleic acid. Further, the nucleic acid probe can hybridize to the target nucleic acid, and includes a cleavage portion, and a signal-generating portion from which a signal is generated, where the cleavage portion is cleaved upon hybridization to the target nucleic acid and generates a first probe fragment and a second probe fragment, at least one of which is capable of dissociating from the target nucleic acid. Further, the nucleic acid probe includes a cleavage portion, which is cleaved upon hybridization to the target nucleic acid, and has formed a locked structure as a result of intramolecular hybridization of the portions of the nucleic acid probe to each other until the nucleic acid probe hybridizes to the target nucleic acid.

  本发明的核酸探针是一种高灵敏、高准确、高快速检测痕量靶核酸的核酸探针，其设计使核酸探针与靶核酸杂交后发生构象变化，发生构象变化的核酸探针杂交结合强度降低，并与靶核酸解离。进一步地，核酸探针可与靶核酸杂交，并包括裂解部分和产生信号的信号产生部分，其中裂解部分在与靶核酸杂交时被裂解，并产生第一探针片段和第二探针片段，其中至少一个片段能够与靶核酸解离。此外，核酸探针还包括裂解部分，裂解部分在与靶核酸杂交时被裂解，并且在核酸探针与靶核酸杂交之前，由于核酸探针各部分之间的分子内杂交形成了锁定结构。
• Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
  申请人：Kool Todd Eric

  公开号：US20060199192A1

  公开（公告）日：2006-09-07

  A universal linker structure is provided, in which a functional group and activating leaving group are placed on a tether, allowing the placement of an electrophile at the end of any nucleic acid sequence. The electrophile on the tether can react with a second nucleic acid carrying a nucleophile when the two nucleic acids are hybridized near one another, resulting in release of the leaving group, and creation of a functional change. The linker can be designed to destabilize the ligation product without slowing the rate of reaction. This lowers product inhibition, and the target DNA or RNA can become a catalyst for isothermally generating multiple signals for detection. This enhanced signal is demonstrated in solution experiments and in solid supported assays. The universal linkers of the present invention are simple and inexpensive to prepare, and can be appended to any polynucleotide in automated steps on a standard DNA synthesizer.

  本发明提供了一种通用连接体结构，其中一个官能团和活化离去基团被置于系链上，从而可以在任何核酸序列的末端放置亲电子体。当两个核酸杂交到彼此附近时，系链上的亲电子可与携带亲核剂的第二个核酸发生反应，从而释放离去基团并产生功能变化。连接体的设计可以在不减慢反应速度的情况下破坏连接产物的稳定性。这样可以降低对产物的抑制，目标 DNA 或 RNA 可以成为催化剂，以等温方式产生多个检测信号。这种增强的信号在溶液实验和固体支持的检测中都得到了证实。本发明的通用连接体制备简单、成本低廉，可在标准 DNA 合成器上通过自动步骤添加到任何多核苷酸中。
• DETECTION OF CHEMICAL LIGATION USING FLUORESCENCE QUENCHING LEAVING GROUPS
  申请人：The Board of Trustees of the Leland Stanford Junior University

  公开号：EP1543156A2

  公开（公告）日：2005-06-22