bzC(nBzl)p^obzA(nBzl)p(NHPh)2 | 73706-14-6

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 核糖核苷3'-磷酸盐
• 英文名称: bzC(nBzl)p^obzA(nBzl)p(NHPh)2
• CAS: 73706-14-6
• 化学式: C₆₅H₅₇ClN₁₂O₁₈P₂
• 分子量: 1391.64
• InChiKey: NUIXNXWFHAWDLF-IMVXXVDYSA-N

反应信息

• 作为反应物：
  描述：

  bzC(nBzl)p^obzA(nBzl)p(NHPh)2 、 triethylammonium salt of 2'-O-(o-nitrobenzyl)-5'-O-monomethoxytrityl-N-benzoylcytidine 3'-p-chlorophenyl phosphate 在 mesitylenesulfonyl 1,2,4-triazolide 作用下， 以 吡啶 为溶剂， 生成 (MeOTr)bzC(nBzl)p^obzC(nBzl)p^obzA(nBzl)p(NHPh)2
  参考文献：
  名称：

  转移核糖核酸及其相关化合物的研究。XXXIX。对应于大肠杆菌tRNAfMet 3'端的七聚体和十六碳氢核苷酸的化学合成。

  摘要：

  A heptadecanucleotide corresponding to the 3'-end (bases 61-77) of the tRNAMetf of E.coli was synthesized by the phosphotriester method involving condensation of protected oligonucleotides with chain lengths of 3 to 6. 2'-O-(o-Nitrobenzyl) derivatives of N-acyl-5'-O-monomethoxytrityl nucleosides were used as the starting materials. To protect the 3'-end of the heptadecanucleotide with phosphorodianilidate during the synthesis, protected AACCAp having 3'-phosphorodianilidate was used as the starting nucleotide block. Condensation of protected CCCCGCp or successive condensations of protected CCCp and CGCp gave the protected undecamer in satisfactory yields. The final condensation of the undecamer to protected UCCGGCp yielded the protected heptadecamer, and the product was deprotected to give the 3'-phosphorylated heptadecanucleotide UCCGGCCCCCGAACCAp. A hexadecanucleotide corresponding to the 3'-end of the same tRNAMetf was also synthesized by the phosphotriester method by condensation of the undecamer with protected CCGGCp. The hexadecamer was deblocked and purified by chromatography on ion-exchange and reverse phase supports. This deprotected hexadecanucleotide CCGGCCCCCGCAACCAp (corresponding to bases 62-77) can be joined to the previously synthesized heptamer corresponding to the eukaryotic loop VI sequences to construct a hybrid tRNA.

  DOI：

  10.1248/cpb.29.2799
• 作为产物：
  描述：

  (MeOTr)bzC(nBzl)p^obzA(nBzl)p(NHPh)2 在 溶剂黄146 作用下， 反应 24.0h， 以3.68 g的产率得到bzC(nBzl)p^obzA(nBzl)p(NHPh)2
  参考文献：
  名称：

  转移核糖核酸及其相关化合物的研究。XXXIX。对应于大肠杆菌tRNAfMet 3'端的七聚体和十六碳氢核苷酸的化学合成。

  摘要：

  A heptadecanucleotide corresponding to the 3'-end (bases 61-77) of the tRNAMetf of E.coli was synthesized by the phosphotriester method involving condensation of protected oligonucleotides with chain lengths of 3 to 6. 2'-O-(o-Nitrobenzyl) derivatives of N-acyl-5'-O-monomethoxytrityl nucleosides were used as the starting materials. To protect the 3'-end of the heptadecanucleotide with phosphorodianilidate during the synthesis, protected AACCAp having 3'-phosphorodianilidate was used as the starting nucleotide block. Condensation of protected CCCCGCp or successive condensations of protected CCCp and CGCp gave the protected undecamer in satisfactory yields. The final condensation of the undecamer to protected UCCGGCp yielded the protected heptadecamer, and the product was deprotected to give the 3'-phosphorylated heptadecanucleotide UCCGGCCCCCGAACCAp. A hexadecanucleotide corresponding to the 3'-end of the same tRNAMetf was also synthesized by the phosphotriester method by condensation of the undecamer with protected CCGGCp. The hexadecamer was deblocked and purified by chromatography on ion-exchange and reverse phase supports. This deprotected hexadecanucleotide CCGGCCCCCGCAACCAp (corresponding to bases 62-77) can be joined to the previously synthesized heptamer corresponding to the eukaryotic loop VI sequences to construct a hybrid tRNA.

  DOI：

  10.1248/cpb.29.2799