L-组氨醇磷酸酯 | 25679-93-0

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 中文名称: L-组氨醇磷酸酯
• 英文名称: L-histidinol 1-phosphate
• 英文别名: L-Histidinol-phosphat；L-histidinol phosphate；phosphoric acid mono-[2-amino-3-(1(3)H-imidazol-4-yl)-propyl] ester；[(2S)-2-amino-3-(1H-imidazol-5-yl)propyl] dihydrogen phosphate
• CAS: 25679-93-0
• 化学式: C₆H₁₂N₃O₄P
• 分子量: 221.153
• InChiKey: CWNDERHTHMWBSI-YFKPBYRVSA-N

物化性质

• 沸点：
  596.8±60.0 °C(Predicted)
• 密度：
  1.581±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -4.6
• 重原子数：
  14
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  122
• 氢给体数：
  4
• 氢受体数：
  6

安全信息

• 海关编码：
  2933290090

反应信息

• 作为反应物：
  描述：

  L-组氨醇磷酸酯 在 L-histidinol-phosphatephosphohydrolase 作用下， 以 水 为溶剂， 生成 L-组氨醇
  参考文献：
  名称：

  （βα）8-桶酶随机库的文库选择导致基因表达的意外诱导。

  摘要：

  通过结合体内随机诱变和选择的方法，测试了经常遇到的（βα ）8桶折叠获得新功能的潜力。为此，对编码52种不同的磷酸盐结合（βα ）8桶蛋白的基因进行易错PCR，并将其克隆到表达质粒中。所得的混合库用于转化不同的营养缺陷型大肠杆菌菌株，每个菌株均缺乏具有含磷酸盐底物的酶。将不同的转化子铺板在基本培养基上后，仅观察到两种菌株的生长，这两种菌株都缺少丝氨酸磷酸酶SerB或磷酸丝氨酸氨基转移酶SerC的基因。大肠杆菌的相同突变体基因楠E（编码推定Ñ -acetylmannosamine -6-磷酸2-差向异构酶）和PDX Ĵ（编码吡哆醇5'-磷酸合酶）负责抢救两个Δ SER B和Δ SER C.出乎意料的是，补充NANE和PdxJ的变种不能在体外催化SerB或SerC反应。取而代之的是，RT-qPCR，RNAseq和转录组分析表明，它们通过寻求内源性大肠杆菌的帮助而挽救了这些缺失。通过组氨酸操纵子

  DOI：

  10.1021/acs.biochem.9b00579
• 作为产物：
  描述：

  L-histidinol dihydrochloride 在 barium(II) hydroxide 作用下， 以 水 为溶剂， 反应 1.0h， 以300 mg的产率得到L-组氨醇磷酸酯
  参考文献：
  名称：

  Divergence of Biochemical Function in the HAD Superfamily: d-glycero-d-manno-Heptose-1,7-bisphosphate Phosphatase (GmhB)

  摘要：

  D-glycero-D-manno-Heptose-1,7-bisphosphate phosphatase (GmhB) is a member of the histidinol-phosphate phosphatase (HisB) subfamily of the haloalkanoic acid dehalogenase (HAD) enzyme superfamily. GmhB supports two divergent biochemical pathways in bacteria: the D-glycero-D-manno-heptose-1 alpha-GDP pathway (in S-layer glycoprotein biosynthesis) and the L-glycero-D-manno-heptose-1 beta-ADP pathway (in lipid A biosynthesis). Herein, we report the comparative analysis Of Substrate recognition in selected GmhB orthologs. The substrate specificity of the L-glycero-D-manno-heptose-1 beta-ADP pathway GmhB from Escherichia coli K-12 was evaluated using hexose and heptose bisphosphates, histidinol phosphate, and common organophosphate metabolites. Only D-glycero-D-manno-heptose 1 beta,7-bisphosphate (k(cat)/k(m) = 7 x 10(6) M-1 s(-1)) and D-glycero-D-manno-heptose 1 alpha,7-bisphosphate (k(cat)/K-m, = 7 x 10(4) M-1 s(-1)) displayed physiologically significant Substrate activity. P-31 NMR analysis demonstrated that E. coli GmhB selectively removes the C(7) phosphate. Steady-state kinetic inhibition studies showed that D-glycero-D-manno-heptose 1 beta-phosphate (K-is = 60 mu M, and K-ii = 150 mu M) and histidinol phosphate (K-is = 1 mM, and K-ii = 6 mM), while not hydrolyzed, do in fact bind to E. coli GmhB, which leads to the conclusion that nonproductive binding contributes to substrate discrimination. High catalytic efficiency and a narrow substrate range are characteristic of a well-evolved metabolic enzyme, and as such, E. coli GmhB is set apart from most HAD phosphatases (which are typically inefficient and promiscuous). The specialization of the biochemical function of GmhB was examined by measuring the kinetic constants for hydrolysis of the alpha- and beta-anomers of D-glycero-D-manno-heptose 1 beta,7-bisphosphate catalyzed by the GmhB orthologs of the L-glycero-D-manno- 1 beta-ADP pathways operative in Bordetella bronchiseptica and Mesorhizobium and by the GmhB of the D-glycero-D-manno-heptose 1 alpha-GDP pathway operative in Bacteroides thetaiotaomicron. The results show that although each of these representatives possesses physiologically significant catalytic activity toward both anomers, each displays substantial anomeric specificity. Like E. coli GmhB, B. bronchiseptica GmhB and M. loti GmhB prefer the beta-anomer, whereas B. thetaiotaomicron GmhB is selective for the alpha-anomer. By determining the anomeric configuration of the physiological Substrate (D-glycero-D-manno-heptose 1,7- for each of the four GmhB orthologs, we discovered that the anomeric specificity of GmhB correlates with that of the pathway kinase. The conclusion drawn from this finding is that the evolution of the ancestor to GmhB in the HisB subfamily provided for specialization toward two distinct biochemical functions.

  DOI：

  10.1021/bi902018y

文献信息

• [EN] CELLULAR TRANSPORT SYSTEM FOR TRANSFERRING A SULFONIC ACID CONSTRUCT CARRYING A CARGO INTO THE CYTOPLASM OF A CELL<br/>[FR] SYSTÈME DE TRANSPORT CELLULAIRE PERMETTANT DE TRANSFÉRER UNE CONSTRUCTION D'ACIDE SULFONIQUE PORTANT UN CARGO DANS LE CYTOPLASME D'UNE CELLULE
  申请人：SCIENTIST OF FORTUNE SA

  公开号：WO2019068726A1

  公开（公告）日：2019-04-11

  The present invention relates to a cellular transport system for bringing a sulfonic acid construct which carries a cargo into a cell and releasing the cargo in the cell's cytoplasm, the cellular transport system comprising: (i) a sulfonate transporter located in the cytoplasm membrane of the cell wherein said sulfonate transporter is capable of transporting said sulfonic acid construct across the cytoplasm membrane into the cytoplasm; (ii) a γ-glutamyl transferase (GGT; EC 2.3.2.2) which is modified to be located in the cytoplasm of the cell, wherein said γ-glutamyl transferase is capable of hydrolyzing said sulfonic acid construct so as to release the cargo. Moreover, the present invention relates to the use of a cellular transport system for bringing a sulfonic acid construct which contains a cargo into a cell and releasing the cargo in the cell's cytoplasm. Further, the present invention relates to a γ-glutamyl transferase for hydrolyzing a sulfonic acid construct which contains a cargo.

  本发明涉及一种细胞运输系统，用于将携带货物的磺酸构造体带入细胞并在细胞质中释放货物，所述细胞运输系统包括：（i）位于细胞质膜中的磺酸转运蛋白，其中所述磺酸转运蛋白能够将所述磺酸构造体跨越细胞质膜运输至细胞质；（ii）改性为位于细胞质中的γ-谷氨酰转移酶（GGT；EC 2.3.2.2），其中所述γ-谷氨酰转移酶能够水解所述磺酸构造体以释放货物。此外，本发明涉及使用细胞运输系统将携带货物的磺酸构造体带入细胞并在细胞质中释放货物。此外，本发明涉及用于水解携带货物的磺酸构造体的γ-谷氨酰转移酶。
• Transferases and Oxidoreductases, Nucleic Acids Encoding Them and Methods for Making and Using Them.
  申请人：VERENIUM CORPORATION

  公开号：US20140165221A1

  公开（公告）日：2014-06-12

  This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., d-amino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H 2 O+acceptor<=>a 2-oxo acid+NH 3 +reduced acceptor.

  本发明涉及酶、编码酶的多核苷酸、使用这种多核苷酸和多肽以及更具体地涉及具有转移酶活性（例如转氨酶活性，例如d-氨基酸转移酶活性）和/或氧化还原酶活性（例如脱氢酶活性，例如d-氨基酸脱氢酶活性）的酶，以及催化化学基团的转移、催化转氨作用、催化反应：D-丙氨酸+2-氧戊二酸<=>丙酮酸+D-谷氨酸，和/或催化氧化还原反应、催化氢原子的去除、和/或催化反应：D-氨基酸+H2O+受体<=>2-酮酸+NH3+还原受体。
• Transferases and Oxidoreductases, Nucleic Acids Encoding them and methods for making and using them
  申请人：Weiner David P.

  公开号：US20110033391A1

  公开（公告）日：2011-02-10

  This invention relates generally to enzymes, polynucleotides encoding the enzymes, the use of such polynucleotides and polypeptides and more specifically to enzymes having transferase activity, e.g., transaminase activity, e.g., d-amino-acid transferase activity, and/or oxidoreductase activity, e.g., dehydrogenase activity, e.g., damino-acid dehydrogenase activity, and/or catalyze the transfer of a chemical group, catalyze transamination, catalyze the reaction: D-alanine+2-oxoglutarate<=>pyruvate+D-glutamate, and/or catalyze an oxidation-reduction reaction, catalyze the removal of hydrogen atoms, and/or catalyze the reaction: D-amino acid+H 2 O+acceptor<=>a 2-oxo acid+NH 3 +reduced acceptor. Thus, the invention provides enzymes, compositions, methods for production of pharmaceutical compositions, pharmaceutical intermediates, antibiotics, sweeteners, peptide enzymes, peptide hormones, fuel and fuel additive compositions, foods and food additives, beverage and beverage additives, feeds and feed additives, drugs and drug additives, dietary supplements, textiles, wood, paper, pulp, and detergents comprising the polypeptides or polynucleotides in accordance with the invention.

  本发明涉及酶、编码该酶的多核苷酸、使用这样的多核苷酸和多肽的方法，更具体地涉及具有转移酶活性的酶，例如转氨酶活性，例如d-氨基酸转移酶活性，和/或氧化还原酶活性，例如脱氢酶活性，例如d-氨基酸脱氢酶活性，并且/或催化化学基团的转移，催化转氨作用，催化反应：D-丙氨酸+2-酮戊二酸<=>丙酮酸+D-谷氨酸，并且/或催化氧化还原反应，催化氢原子的去除，并且/或催化反应：D-氨基酸+H2O+受体<=>2-酮酸+NH3+还原受体。因此，本发明提供了酶、组合物、生产药物组合物、药物中间体、抗生素、甜味剂、肽酶、肽激素、燃料和燃料添加剂组合物、食品和食品添加剂、饮料和饮料添加剂、饲料和饲料添加剂、药品和药品添加剂、膳食补充剂、纺织品、木材、纸浆和洗涤剂，其中包括根据本发明的多肽或多核苷酸。
• Herbicide-tolerant plants through bypassing metabolic pathway
  申请人：Zink Olivier

  公开号：US20080163392A1

  公开（公告）日：2008-07-03

  The invention concerns a novel method for making herbicide-tolerant plants, in particular to HPPD inhibiting herbicides, the nucleic acid sequences coding for enzymes capable of being used in said method, expression cassettes containing them and transgenic plants comprising at least one of said expression cassettes.

  该发明涉及一种制备耐除草剂植物的新方法，特别是针对HPPD抑制剂除草剂，涉及编码能够在所述方法中使用的酶的核酸序列，包含它们的表达盒和转基因植物，其中至少包含其中一种表达盒。
• Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
  申请人：Ceres Incorporated

  公开号：EP1033405A2

  公开（公告）日：2000-09-06

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. 0Arabidopsis DNA is used in the present experiment, but the procedure is a general one.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体、或对具有共同性状的生物群体进行聚类的工具。 本实验中使用的是拟南芥 DNA，但这是一个通用程序。