二磷酸酚酞 | 2090-82-6

• 物质功能分类: 无机化工 - 无机盐 - 磷化物、金属的磷酸、偏磷酸、次磷酸和焦磷酸盐
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 中文名称: 二磷酸酚酞
• 中文别名: 酚酞二磷酸
• 英文名称: Phenolphthalein diphosphate
• 英文别名: [4-[3-oxo-1-(4-phosphonooxyphenyl)-2-benzofuran-1-yl]phenyl] dihydrogen phosphate
• CAS: 2090-82-6
• 化学式: C₂₀H₁₆O₁₀P₂
• 分子量: 478.3
• InChiKey: WMDDNKROYKCDJC-UHFFFAOYSA-N

物化性质

• 熔点：
  198 °C
• 沸点：
  762.1±70.0 °C(Predicted)
• 密度：
  1.671±0.06 g/cm3(Predicted)
• 稳定性/保质期：

  常温常压下保持稳定，应避免与不相容的材料接触。

计算性质

• 辛醇/水分配系数(LogP)：
  1
• 重原子数：
  32
• 可旋转键数：
  6
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  160
• 氢给体数：
  4
• 氢受体数：
  10

安全信息

• 安全说明：
  S24/25
• WGK Germany：
  3
• 海关编码：
  29322985
• 危险品标志：
  Xi

制备方法与用途

用于测定酸性磷酸酶最常用的试剂是二磷酸酚酞或其四钠盐、百里酚酞单磷酸二钠和萘胺单磷酸二钠。这些物质均为经典磷酸盐衍生物，通常以钠盐的形式使用，主要用于确定pH值。在这些试剂中，二磷酸酚酞或其四钠盐更为有效且易于观察。相较于其他试剂，该试剂成本较低，在介质中释放的酚酞毒性也更低。

文献信息

• Dry analytical element for alkaline phosphatase assay
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0132830A2

  公开（公告）日：1985-02-13

  Disclosed herein is a dry analytical element and a method of using same for quantitatively detecting alkaline phosphatase in an aqueous liquid. The element comprises, in fluid contact, first and second reagent zones wherein the first reagent zone contains a substrate for alkaline phosphatase, e.g. p-nitrophenyl phosphate, and the second reagent zone contains a buffer which is an alkali metal or ammonium salt and has a pKa in the range of 9-11.5, e.g. an alkali metal salt of carbonic acid.

  本文公开了一种干式分析元件及其使用方法，用于定量检测水性液体中的碱性磷酸酶。该元件包括流体接触的第一试剂区和第二试剂区，其中第一试剂区含有碱性磷酸酶的底物，例如对硝基苯磷酸，第二试剂区含有缓冲液，缓冲液是碱金属盐或铵盐，pKa 在 9-11.5 之间，例如碳酸的碱金属盐。
• Analytical element and method for theophylline determination having increased alkaline phosphatase isoenzyme
  申请人：EASTMAN KODAK COMPANY (a New Jersey corporation)

  公开号：EP0258035A2

  公开（公告）日：1988-03-02

  Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element contains a suitable buffer and first and second zones in fluid contact. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The isoenzyme is present in one of the zones in an amount of at least 100 I.U./m². This level of isoenzyme reduces or eliminates bias caused by endogenous alkaline phosphatase and hemoglobin during an assay.

  茶碱可以用一种分析元素和方法来测定，这种元素和方法利用茶碱对适当底物上碱性磷酸酶活性的抑制作用。检测在 pH 值为 9 或更低的条件下进行。检测元件包含合适的缓冲液以及液体接触的第一区和第二区。碱性磷酸酶同工酶和该同工酶的适当底物位于该元件的不同区域。其中一个区中的同工酶含量至少为 100 I.U./m²。这种水平的同工酶可减少或消除化验过程中内源性碱性磷酸酶和血红蛋白造成的偏差。
• Analytical element for theophylline determination using buffer in a subbing zone
  申请人：EASTMAN KODAK COMPANY

  公开号：EP0353026A2

  公开（公告）日：1990-01-31

  Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises a porous spreading zone, a subbing zone and at least one other zone. The zones are in fluid contact with each other. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The subbing zone contains polyvinylpyrroli­done and a buffer for maintaining a pH of 9 or less.

  茶碱可以用一种分析元素和方法来测定，这种元素和方法利用茶碱对适当底物上碱性磷酸酶活性的抑制作用。检测在 pH 值为 9 或更低的条件下进行。该元件包括一个多孔扩散区、一个吸附区和至少一个其他区。这些区彼此流体接触。碱性磷酸酶同工酶和该同工酶的合适底物位于该元件的不同区域。吸附区含有聚乙烯吡咯烷酮和缓冲液，用于保持 pH 值在 9 或以下。
• Gel electrophoresis
  申请人：Bissbort, Siegbert Heinrich

  公开号：EP0663593A2

  公开（公告）日：1995-07-19

  Method of establishing the position, in a gel, of a compound separated in the gel from one or more other compounds by gel electrophoresis. The method comprises the step of moistening a dry inert sorbent substrate, which has been pre-impregnated with a constituent forming part of a detection agent, to impregnate the substrate in moist form with the detection agent. The method also comprises the step of bringing the moistened substrate into contact with the gel containing said compound separated in the gel by electrophoresis. The separated compound in the gel is allowed to react with said detection agent, to cause a detectable change at a detectable position in the gel or on the substrate with the position of the detectable change in the gel or on the substrate providing an indication of the position of the separated compound in the gel.

  通过凝胶电泳确定在凝胶中与一种或多种其他化合物分离的化合物的位置的方法。该方法包括以下步骤：湿润干燥的惰性吸附剂基质，该基质已用构成检测剂一部分的成分预先浸渍过，使基质在湿润状态下浸渍检测剂。该方法还包括以下步骤：将湿润的基质与凝胶接触，凝胶中含有通过电泳分离的所述化合物。让凝胶中分离的化合物与所述检测剂反应，使凝胶中或基质上的可检测位置发生可检测的变化，凝胶中或基质上可检测变化的位置提供了凝胶中分离化合物位置的指示。
• POLYMER ELECTROLYTE COMPOSITION AND POLYMER ELECTROLYTE MEMBRANE, MEMBRANE-ELECTROLYTE ASSEMBLY, AND SOLID POLYMER FUEL CELL USING SAME
  申请人：Toray Industries, Inc.

  公开号：EP3131145A1

  公开（公告）日：2017-02-15

  [Problem] To provide: a polymer electrolyte composition that has excellent chemical stability that makes it possible to withstand a strong oxidizing atmosphere during fuel cell operation, that makes it possible to achieve excellent protonic conductivity under low humidity conditions, excellent mechanical strength, and excellent physical durability, and that is highly practical; and a polymer electrolyte membrane, a membrane-electrode assembly, and a solid polymer fuel cell that use the polymer electrolyte composition. [Solution] A polymer electrolyte composition that comprises at least an ionic group-containing polymer (A), an organic phosphorus-based additive (C), and a nitrogen-containing heteroaromatic ring system additive (D) and that is characterized by the nitrogen-containing heteroaromatic ring system additive (D) comprising at least three nitrogen-containing heteroaromatic rings within a molecule thereof. In addition, the polymer electrolyte membrane, the catalyst layer-equipped electrolyte membrane, the membrane-electrode assembly, and the solid polymer fuel cell of the present invention are characterized by being configured using the polymer electrolyte composition.

  问题 提供一种聚合物电解质组合物，该组合物具有优异的化学稳定性，能够在燃料电池运行期间耐受强氧化性气氛，能够在低湿度条件下实现优异的质子电导率，具有优异的机械强度和优异的物理耐久性，并且具有很高的实用性；以及一种使用该聚合物电解质组合物的聚合物电解质膜、膜电极组件和固体聚合物燃料电池。[解决方案]一种聚合物电解质组合物，它至少包含一种含离子基团的聚合物（A）、一种有机磷基添加剂（C）和一种含氮杂芳香族环系添加剂（D），其特征在于含氮杂芳香族环系添加剂（D）在其分子内至少包含三个含氮杂芳香族环。此外，本发明的聚合物电解质膜、配备催化剂层的电解质膜、膜电极组件和固体聚合物燃料电池的特征在于使用聚合物电解质组合物进行配置。