1-amino-17-N-(biotinylamido)-3,6,9,12,15-pentaoxaheptadecane

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 噻吩并咪唑类化合物
• 英文名称: 1-amino-17-N-(biotinylamido)-3,6,9,12,15-pentaoxaheptadecane
• 英文别名: (3'aS,6'aR,7S)-spiro[1,15,18,21,24-pentaoxa-4,5-diazacyclohexacosane-7,4'-3,3a,6,6a-tetrahydro-1H-thieno[3,4-d]imidazole]-2',6,12-trione
• 化学式: C₂₃H₄₀N₄O₈S
• 分子量: 532.659
• InChiKey: GVHSUCSBODALBO-JTAQYXEDSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.5
• 重原子数：
  36
• 可旋转键数：
  0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.87
• 拓扑面积：
  171
• 氢给体数：
  4
• 氢受体数：
  10

反应信息

• 作为产物：
  描述：

  1CHCl3 -CH3 OH-concentrated NH4 OH 、 N-oxysuccinimidyl biotin 、 异丙醚 、 1,17-二氨基-3,6,9,12,15-五氧杂十七烷 生成 1-amino-17-N-(biotinylamido)-3,6,9,12,15-pentaoxaheptadecane
  参考文献：
  名称：

  摘要：

  DOI：

文献信息

• Sample processing to release nucleic acids for direct detection
  申请人：——

  公开号：US20010031473A1

  公开（公告）日：2001-10-18

  The present invention describes compositions and methods for releasing nucleic acids from cells in a form that is suitable for labeling/capture, amplification, or detection in a single reagent addition step. The compositions include a lipid, membrane fluidizing compound, enzyme for degrading cell structure, metal chelators, or one or more nucleic acid probes or primers complementary to the nucleic acid to be detected. The compositions are non-denaturing and non-inhibitory of enzymes or proteins that are used in nucleic acid release, amplification, labeling or detection. The invention also provides kits for performing the above methods.

  本发明描述了一种将核酸从细胞中释放出来并以适合于标记/捕获、扩增或检测的形式的组合物和方法，该方法只需要一次试剂添加步骤。该组合物包括脂质、膜流动化合物、降解细胞结构的酶、金属螯合剂或与待检测的核酸互补的一个或多个核酸探针或引物。该组合物是非变性的，不会抑制用于核酸释放、扩增、标记或检测的酶或蛋白质。本发明还提供了用于执行上述方法的试剂盒。
• Stabilization of nucleic acid amplification cocktails
  申请人：——

  公开号：US20020061537A1

  公开（公告）日：2002-05-23

  The present invention provides a cocktail of reagents for nucleic acid amplification that are stabilized by inclusion of a reversible inhibitor of undesirable reactions. Such cocktail of reagents eliminates the requirement for separate preparation and quality control of each reagent used in a reaction. Methods to prepare stabilized cocktails and to use stabilized cocktails also are included. The stabilized cocktail compositions also can include reagents to release nucleic acid from cells and to label the nucleic acid, allowing detection of nucleic acid in a sample with a single reagent addition step. The invention also provides kits for performing the above methods.

  本发明提供了一种核酸扩增试剂混合物，其通过包含可逆抑制剂来稳定，以防止不良反应的发生。这种试剂混合物消除了在反应中使用的每种试剂的单独制备和质量控制的要求。还包括制备稳定混合物和使用稳定混合物的方法。稳定的混合物组成还可以包括释放细胞中核酸和标记核酸的试剂，使得在样品中检测核酸只需要一个试剂添加步骤。本发明还提供了执行上述方法的试剂盒。
• Eucaryotic genomic dna dot-blot hybridization method
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0228075A2

  公开（公告）日：1987-07-08

  A dot-blot hybridization method and test kit for detecting normal and mutated genes in unfractionated eucaryotic genomic DNA. Unfractionated single stranded sample DNA is immobilized in a limited zone on a solid support sheet and hybridized with an oligonucleotide probe complementary to the sequence of interest. After stringency washing to remove nonspecifically hybridized probe, the dot-blot zone is examined for the presence of hybridized probe, usually by detection of a label attached to the probe. Hybridization conditions are selected to provide a level of detectable hybridized probe in the dot-blot zone which is readily distinguishable from any background signal associated with the surrounding area on the support sheet. The method eliminates the need for substantial purification and fragmentation of sample DNA required by prior art techniques.

  一种点印迹杂交方法和检测试剂盒，用于检测未分馏的真核基因组 DNA 中的正常基因和突变基因。将未分馏的单链样本 DNA 固定在固体支持片上的有限区域内，然后与与相关序列互补的寡核苷酸探针杂交。经过严格洗涤去除非特异性杂交探针后，检查点印迹区是否存在杂交探针，通常是通过检测连接到探针上的标签。选择杂交条件的目的是使点印迹区可检测到的杂交探针水平能与支持片上周围区域的背景信号区分开来。该方法无需对现有技术所需的样本 DNA 进行大量纯化和破碎。
• Rapid detection of nucleic acid sequences in a sample by labeling the sample
  申请人：MILES INC.

  公开号：EP0235726A2

  公开（公告）日：1987-09-09

  A method for detecting one or more microorganisms or polynucleotide sequences from eukaroytic sources in a nucleic acid-containing test sample comprising (a) preparing a test sample comprising labeling the nucleic acids in the test sample, (b) preparing one or more process by immobilizing a single-stranded nucleic acid of one or more known microorganisms or sequences from eukaroytic sources, (c) contacting, under hybridization conditions, the labeled single-stranded nucleic acid to form hybridized labeled nucleic acids, and (d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia.

  一种检测含核酸检测样本中一种或多种微生物或真核生物来源的多核苷酸序列的方法，包括 (a) 制备测试样品，包括标记测试样品中的核酸、 (b) 通过固定一种或多种已知微生物的单链核酸或真核来源的核酸序列，制备一 个或多个过程、 (c) 在杂交条件下，与标记的单链核酸接触，形成杂交标记核酸，以及 (d) 通过检测标记来检测杂交核酸。该方法可用于检测遗传疾病，如镰状细胞性贫血。
• Assay for nucleic acid sequences in a sample
  申请人：Bayer Corporation

  公开号：EP0281927A2

  公开（公告）日：1988-09-14

  A method for detecting (i) one or more microorganisms or (ii) nucleic acid sequences from a prokaryotic source or an eukaryotic source in a nucleic acid-containing test sample comprising (a) labeling the nucleic acids in the test sample, (b) contacting, under hybridization conditions, the labeled hybridizable nucleic acid and one or more immobilized hybridizable nucleic acid probes comprising (i) one or more known microorganisms or (ii) sequences from eukaroytic or prokaryotic sources, to form hybridized labeled nucleic acids, and (d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia.

  一种检测含核酸检测样本中 (i) 一种或多种微生物或 (ii) 来自原核生物源或真核生物源的核酸序列的方法，包括 (a) 标记测试样本中的核酸、 (b) 在杂交条件下，将标记的可杂交核酸与一个或多个固定的可杂交核酸探针（包括 (i) 一个或多个已知微生物或 (ii) 来自真核或原核来源的序列）接触，以形成杂交标记核酸，和 (c) 在杂交条件下，将标记的可杂交核酸与一个或多个固定的可杂交核酸探针（包括 (i) 一个或多个已知微生物或 (ii) 来自真核或原核来源的序列）接触，以形成杂交标记核酸，和 (d) 通过检测标记对杂交核酸进行检测。该方法可用于检测遗传疾病，如镰状细胞性贫血。