[4-(4,4,4-Trifluoro-3-oxobutyl)phenyl] dihydrogen phosphate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 英文名称: [4-(4,4,4-Trifluoro-3-oxobutyl)phenyl] dihydrogen phosphate
• 化学式: C₁₀H₁₀F₃O₅P
• 分子量: 298.15
• InChiKey: ZIPMASRZLPIZKB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.3
• 重原子数：
  19
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.3
• 拓扑面积：
  83.8
• 氢给体数：
  2
• 氢受体数：
  8

文献信息

• Signal enhancement in immunoassay by inhibition of enzymatic catalysis
  申请人：Becton Dickinson and Company

  公开号：EP0271731A2

  公开（公告）日：1988-06-22

  A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.

  一种对怀疑存在于液体样品中的配体进行酶免疫测定的方法，包括使用至少两种酶和其中一种酶的阻断调节剂进行信号放大。 液体中存在的配体与抗配体和酶标记的示踪剂结合。 由此产生的结合部分被分离，示踪剂中的酶从阻断调节剂中去除阻断基团。 调节剂激活或抑制第二种酶，该酶催化底物向产物的转化。 液体中配体的存在与否通过与产物相关的信号（如颜色变化或颜色变化率）来指示。 本发明包括一类新的酶抑制剂和阻断抑制剂，以及一套用于实施本发明方法的材料。
• Signal enhancement in assay for an enzyme
  申请人：Becton Dickinson and Company

  公开号：EP0354548A2

  公开（公告）日：1990-02-14

  A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the invention.

  一种检测液体中疑似存在的未知酶的方法，包括使用第二种酶和第二种酶的阻断调制剂进行信号放大。液体中的未知酶会从阻断调制剂中去除阻断基团。由此产生的调制剂激活或抑制第二种酶，第二种酶催化指示剂反应，其中底物转化为产物。液体中未知酶的存在与否可通过与指示剂反应相关的信号（如颜色变化或颜色变化率）来显示。样品中酶的浓度可通过测量信号来确定。本发明包括一套用于实施本发明方法的材料。
• Immunoassay on a preblocked solid surface
  申请人：Becton Dickinson and Company

  公开号：EP0369361A2

  公开（公告）日：1990-05-23

  A method for immunoassay of a viral antigen is performed on a membrane precoated with an inert protein. Nonimmunological capture of antigen takes place by absorption onto the coated membrane. Captured antigen binds to a tracer which includes a label conjugated to a specific antibody, the inert protein concomitantly inhibiting nonspecific binding of tracer. The label may be an enzyme which converts a substrate to a detectable product or converts a blocked inhibitor to an inhibitor whereby a second enzyme is inhibited from converting a substrate to a product. The invention includes a kit of materials for performing an assay in accordance with the method of the invention.

  病毒抗原的免疫测定方法是在预先涂有惰性蛋白质的膜上进行的。抗原通过被涂膜吸收而被非免疫捕获。捕获的抗原与示踪剂结合，示踪剂包括与特异性抗体结合的标签，惰性蛋白同时抑制示踪剂的非特异性结合。标签可以是一种酶，它能将底物转化为可检测的产物，或将被阻断的抑制剂转化为抑制剂，从而抑制第二种酶将底物转化为产物。本发明包括一套材料，用于按照本发明的方法进行检测。
• Chromogenic substrate for esterase and immunoassay using same
  申请人：Becton Dickinson and Company

  公开号：EP0369657A2

  公开（公告）日：1990-05-23

  A chromogenic substrate for an esterase, 4-[(3,5-dichloro-4-butyryloxyphenyl)imino]-2,5-cyclo­hexadien-1-one, is useful in an assay for determination of the presence of concentration of an enzyme or a ligand in a liquid. The assay may be an ELISA using the substrate or a dual enzyme assay including a blocked inhibitor which is cleaved by a hydrolase to an inhibitor of the esterase. The invention includes a kit of materials including the substrate for performing an assay.

  4-[(3,5-dichloro-4-butyryloxyphenyl)imino]-2,5-cyclohexadien-1-one 是一种酯酶的发色底物，可用于测定液体中酶或配体的存在浓度。该测定法可以是使用底物的酶联免疫吸附测定法，也可以是双酶测定法，包括被水解酶裂解成酯酶抑制剂的阻断抑制剂。本发明包括一种材料试剂盒，其中包括用于进行测定的底物。
• Fluoroketones as blocked enzyme inhibitors for use in immunoassays
  申请人：Becton Dickinson and Company

  公开号：EP0501526B1

  公开（公告）日：1996-02-28