[2-Chloro-5-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 英文名称: [2-Chloro-5-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
• 化学式: C₁₈H₂₁Cl₂O₇P
• 分子量: 451.2
• InChiKey: AGMXMWDSGUBNPT-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.4
• 重原子数：
  28
• 可旋转键数：
  4
• 环数：
  6.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  94.4
• 氢给体数：
  2
• 氢受体数：
  7

文献信息

• Use of 14-3-3 proteins and a method for determining the same in the fluids or tissues of organisms
  申请人：Mueller E G Werner

  公开号：US20050009094A1

  公开（公告）日：2005-01-13

  The object of the present invention is to provide a method for the detection and/or quantification of the 14-3-3 proteins or their isoforms for early stage diagnosis of TSE-diseases, which method allows to perform the diagnosis in the living organism. It is furthermore an object, to detect a contamination of the sample by the parallel determination of a second antigen. This object according to the invention is solved by making use of the biochemical characteristics of the members of the 14-3-3 protein family, which bind to specific amino acid motifs like X(n)-XSXXSXXSX-X(n) or to the motif RSXpSXP (SEQ ID NO: 12) within peptides or proteins. For determining one or more isoforms or the entirety of the 14-3-3 protein(s) and for specific binding, one uses modified solid phases like e.g. microtiter plates, which are coated with a synthetic or natural peptide containing a binding motif for 14-3-3 proteins, e.g. a chemically synthesised peptide having the motif CAALPKINRSApSEPSLHR (SEQ ID NO: 1). After the addition of the extracts or bodily fluids to be investigated the detection and quantification of the generated peptide-14-3-3 protein complexes is accomplished by means of labeled antibodies. The use of the 14-3-3 protein family and/or of individual isoforms of the 14-3-3 proteins according to the invention can be employed as an effect monitor or biomonitor in aquatic invertebrates after environmental effects like the presence of polychlorinated biphenyls (PCBs), (xeno)estrogens etc. Moreover, the method can be used for early diagnosis of TSE-diseases like e.g. Creutzfeldt-Jakob disease (CJD) and its novel form (variant) in young persons (vCJD) and Bovine Spongiform Encephalopathy (BSE) or comparable diseases. Thus a diagnostic marker (surrogate marker) is available, which can be used in the living organism as a screening marker, confirmation marker or single marker.

  本发明的目的是提供一种检测和/或定量 14-3-3 蛋白质或其异构体的方法，以用于 TSE 疾病的早期诊断，该方法可在活体生物体内进行诊断。此外，本发明的另一个目的是通过平行测定第二种抗原来检测样本污染。根据本发明，这一目标可通过利用 14-3-3 蛋白家族成员的生化特性来解决，14-3-3 蛋白与特定的氨基酸基团如 X(n)-XSXXSXXSX-X(n) 或肽或蛋白质中的基团 RSXpSXP（SEQ ID NO：12）结合。为了确定 14-3-3 蛋白质的一种或多种异构体或整个 14-3-3 蛋白质，并进行特异性结合，可使用改性固相，如微孔板，其上涂有含有 14-3-3 蛋白质结合基团的合成肽或天然肽，如具有基团 CAALPKINRSApSEPSLHR（SEQ ID NO: 1）的化学合成肽。加入要研究的提取物或体液后，通过标记的抗体对生成的肽-14-3-3 蛋白复合物进行检测和定量。根据本发明，14-3-3 蛋白家族和/或 14-3-3 蛋白的单个异构体可用作水生无脊椎动物受环境影响（如多氯联苯（PCB）、（异）雌激素等）后的效应监测器或生物监测器。此外，该方法还可用于克雅氏病（CJD）及其在年轻人中的新形式（变体）（vCJD）和牛海绵状脑病（BSE）或类似疾病的早期诊断。因此，诊断标记物（替代标记物）是可用的，可在生物体内用作筛选标记物、确认标记物或单一标记物。
• Verfahren zum Nachweis von Umweltverschmutzung unter Verwendung von 14-3-3 Proteinen als Biomarker
  申请人：MÜLLER, Werner E. G.

  公开号：EP0983377B1

  公开（公告）日：2005-05-18
• 14-3-3 PROTEINE ALS SENSITIVE BIOMARKER FÜR POLLUTION U.A. MIT PCBS UND (XENO)ÖSTROGENEN
  申请人：MÜLLER, Werner E. G.

  公开号：EP0983377A1

  公开（公告）日：2000-03-08
• Reagents, kits and methods for detecting biological molecules by energy transfer from an activated chemiluminescent substrate to an energy acceptor dye
  申请人：Life Technologies Corporation

  公开号：EP2390663B1

  公开（公告）日：2013-11-13
• DOSAGE D'ARSENIC PAR ELISA
  申请人：Université de Lorraine

  公开号：EP2678691B1

  公开（公告）日：2016-07-13