申请人:KYOWA MEDEX CO., LTD.
公开号:EP1767942A1
公开(公告)日:2007-03-28
A method for immunologically determining an analyte in which a nonspecific reaction is suppressed, which comprises the steps of:
allowing an enzyme-labeled antibody in which an enzyme is bound as a label to a first antibody that specifically binds to the analyte, to react with the sample in an aqueous medium to form an immune complex of the analyte and the enzyme-labeled antibody, said enzyme-labeled antibody substantially containing only one or plural number of enzyme-labeled antibodies selected from the group consisting of enzyme-labeled antibodies having a binding ratio between the first antibody and the enzyme of 1:1, 1:2, 1:3 and 2:1, respectively, and measuring the enzyme activity of the immune complex.
一种抑制非特异性反应的免疫测定分析物的方法,它包括以下步骤
让酶标记抗体(其中酶作为标记与特异性结合分析物的第一抗体结合)在水介质中与样品反应,形成分析物与酶标记抗体的免疫复合物,所述酶标记抗体实质上只含有一种或多种酶标记抗体,这些酶标记抗体选自第一抗体与酶的结合比分别为1:1、1:2、1:3和2:1的酶标记抗体组:分别为 1:1、1:2、1:3 和 2:1,并测量免疫复合物的酶活性。