pantothenate kinase | 9026-48-6

• 分子结构分类: 转移酶 - 转移含磷基团 - 以醇基作为受体的磷酸转移酶
• 英文名称: pantothenate kinase
• 英文别名: pantothenate kinase (phosphorylating)；pantothenic acid kinase；ATP:pantothenate 4'-phosphotransferase；D-pantothenate kinase；pantothenate kinase from Staphylococcus aureus
• CAS: 9026-48-6

反应信息

• 作为试剂：
  描述：

  、 5’-三磷酸腺苷 在 dephospho-CoA kinase from Escherichia coli 、 pantothenate kinase 、 phosphopantetheine adenylyltransferase 、 magnesium chloride 作用下， 以 aq. buffer 为溶剂， 反应 3.0h， 以50%的产率得到
  参考文献：
  名称：

  Mechanistic Insight with HBCH₂CoA as a Probe to Polyhydroxybutyrate (PHB) Synthases

  摘要：

  Polyhydroxybutyrate (PHB) synthases catalyze the polymerization of 3-(R)-hydroxybutyrate coenzyme A (HBCoA) to produce polyoxoesters of 1-2 MDa. A substrate analogue HBCH2CoA, in which the S in HBCoA is replaced with a CH2 group, was synthesized in 13 steps using a chemoenzymatic approach in a 7.5% overall yield. Kinetic studies reveal it is a competitive inhibitor of a class I and a class III PHB synthases, with Kis of 40 and 14 μM, respectively. To probe the elongation steps of the polymerization, HBCH2CoA was incubated with a synthase acylated with a [(3)H]-saturated trimer-CoA ([(3)H]-sTCoA). The products of the reaction were shown to be the methylene analogue of [(3)H]-sTCoA ([(3)H]-sT-CH2-CoA), saturated dimer-([(3)H]-sD-CO2H), and trimer-acid ([(3)H]-sT-CO2H), distinct from the expected methylene analogue of [(3)H]-saturated tetramer-CoA ([(3)H]-sTet-CH2-CoA). Detection of [(3)H]-sT-CH2-CoA and its slow rate of formation suggest that HBCH2CoA may be reporting on the termination and repriming process of the synthases, rather than elongation.

  DOI：

  10.1021/cb5002735