4-(氟甲基)苯基磷酸酯 | 152406-98-9

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 有机磷酸及其衍生物
• 中文名称: 4-(氟甲基)苯基磷酸酯
• 中文别名: [4-(氟甲基)苯基]磷酸二氢酯
• 英文名称: 4-(Fluoromethyl)phenyl phosphate
• 英文别名: [4-(fluoromethyl)phenyl] dihydrogen phosphate
• CAS: 152406-98-9
• 化学式: C₇H₈FO₄P
• 分子量: 206.11
• InChiKey: BHOAQALZKIWXQV-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.4
• 重原子数：
  13
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.14
• 拓扑面积：
  66.8
• 氢给体数：
  2
• 氢受体数：
  5

文献信息

• Polypeptide Immobilization
  申请人：The University of Chicago

  公开号：US20150369816A1

  公开（公告）日：2015-12-24

  The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.

  本发明提供了一种方法，包括(a)提供连接到基板上的反应配体；(b)将基板与融合多肽接触，所述融合多肽包括与显示多肽融合的捕获多肽，在条件下，使得所述反应配体共价结合到所述捕获多肽；以及(c)分析所述显示多肽。
• UNNATURAL AMINO ACIDS CAPABLE OF COVALENTLY MODIFYING PROTEIN PHOSPHATASES AND THEIR USE AS GENERAL AND SPECIFIC INHIBITORS AND PROBES
  申请人：Shen Kui

  公开号：US20100061936A1

  公开（公告）日：2010-03-11

  An unnatural amino acid including a phosphate mimicking group for mimicking a phosphate group in phosphoamino acids and a protein phosphatase modifying group for covalently binding protein phosphatases. A probe for detecting disease including a phosphate mimicking group for mimicking a phosphate group in phosphoamino acids and a protein phosphatase modifying group for covalently binding protein phosphatases. A method for detecting the presence of disease by administering the unnatural amino acid, binding the unnatural amino acid with a phosphatase, detecting a signal, and detecting the presence of disease. A method of identifying a known protein phosphatase, and a method of identifying an unknown protein phosphatase. A method of making the unnatural amino acid.

  包括一种模拟磷酸基团的非天然氨基酸，用于模拟磷酸氨基酸中的磷酸基团，以及用于共价结合蛋白磷酸酶的蛋白磷酸酶修饰基团的非天然氨基酸。一种用于检测疾病的探针，包括一种模拟磷酸基团的非天然氨基酸，用于模拟磷酸氨基酸中的磷酸基团，以及用于共价结合蛋白磷酸酶的蛋白磷酸酶修饰基团。一种通过给予非天然氨基酸，结合非天然氨基酸与磷酸酶，检测信号，并检测疾病存在的方法。一种识别已知蛋白磷酸酶的方法，以及一种识别未知蛋白磷酸酶的方法。一种制备非天然氨基酸的方法。
• Compounds, compositions and methods for generating chemiluminescence with phosphatase enzymes
  申请人：——

  公开号：US20030023089A1

  公开（公告）日：2003-01-30

  Novel heterocyclic compounds which generate chemiluminescence on reaction with a phosphatase enzyme are provided as well as a process for their preparation and intermediates useful therein. The compounds comprise a nitrogen, oxygen or sulfur-containing heterocyclic ring system bearing an exocyclic carbon-carbon double bond. The double bond is further substituted at the distal carbon with a phosphate group and an oxygen or sulfur atom-containing group. Novel compositions further comprising a cationic aromatic compound (CAC) in addition to the heterocyclic phosphate compound are provided. The addition of the CAC in the composition greatly increases the production of chemiluminescence and provides improved detection sensitivity. Compositions further comprising an anionic surfactant and a non-ionic surfactant provide additional improvements in detection sensitivity. The novel chemiluminescent compounds and compositions are useful in methods for producing light and in assays for phosphatase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.

  本发明提供了产生化学发光的新型杂环化合物，其与磷酸酶酶反应时会产生化学发光，以及用于其制备和有用中间体的方法。该化合物包括带有外向碳-碳双键的氮、氧或硫含杂环环系统。双键在远端碳上进一步取代为一个磷酸酯基团和一个含氧或硫原子的基团。此外，本发明还提供了进一步包括阳离子芳香化合物（CAC）的新型组合物，除了杂环磷酸酯化合物外。在组合物中加入CAC可以大大增加化学发光的产生，并提供改进的检测灵敏度。进一步包括阴离子表面活性剂和非离子表面活性剂的组合物提供了额外的检测灵敏度改进。新型化学发光化合物和组合物在产生光的方法、磷酸酶酶和酶抑制剂的检测方法以及使用酶标记的特异性结合对的检测方法中均有用途。
• Polypeptide immobilization
  申请人：Mrksich Milan

  公开号：US20080064606A1

  公开（公告）日：2008-03-13

  A substrate comprises a surface, and a plurality of moieties, on at least a portion of the surface. The moieties are moieties of formula: Surf-L-Q-T, where -T comprises a reactant ligand, and Surf- designates where the moiety attaches to the surface. The substrate can be made into a protein chip by the reaction of a reactant ligand and a fusion polypeptide, where the fusion polypeptide includes a capture polypeptide moiety which corresponds to the reactant ligand.

  底物包括一个表面和多个基团，位于表面的至少一部分上。 基团的公式为：Surf-L-Q-T，其中-T包括反应配体，Surf-指示基团连接到表面的位置。 通过反应配体和融合多肽的反应，可以将底物制成蛋白质芯片，其中融合多肽包括与反应配体相对应的捕获多肽基团。
• POLYPEPTIDE IMMOBILIZATION
  申请人：Mrksich Milan

  公开号：US20120142547A1

  公开（公告）日：2012-06-07

  The present invention provides a method, comprising (a) providing a reactant ligand attached to a substrate; (b) contacting the substrate with a fusion polypeptide, said fusion polypeptide comprising a capture polypeptide fused to a display polypeptide under conditions such that said reactant ligand covalently binds to said capture polypeptide; and (c) analyzing said display polypeptide.

  本发明提供了一种方法，包括(a)提供连接到基质上的反应配体；(b)将基质与融合多肽接触，所述融合多肽包括捕获多肽与展示多肽融合，在条件下使所述反应配体共价结合到所述捕获多肽上；(c)分析所述展示多肽。