The invention is directed to enhanced methods for detecting an analyte of interest in situ, by immunoassay, or by hybridization comprising binding an enzyme-labeled conjugate molecule to an analyte of interest in the presence of a redox-inactive reductive species and a soluble metal ion. The enzyme catalyzes the conversion of the inactive reductive species to an active reducing agent, which in turn reduces the metal ion to a metal atom thereby providing an enhanced means of detecting the analyte via metal deposition.
本发明涉及原位、免疫测定或杂交法检测有关分析物的增强方法,包括在氧化还原活性还原 物和可溶性
金属离子存在的情况下,将酶标记的共轭分子与有关分析物结合。酶催化无活性还原物转化为活性还原剂,活性还原剂又将
金属离子还原为
金属原子,从而提供了一种通过
金属沉积检测分析物的增强方法。