sulphatase from Patella vulgata

• 英文名称: sulphatase from Patella vulgata

反应信息

• 作为试剂：
  描述：

  10,13-二甲基-17-氧代-3-磺基氧基-1,2,3,4,7,8,9,11,12,14,15,16-十二氢环戊烯并[a]菲 在 glucuronidase 、 sulphatase from Patella vulgata 咪唑 、 potassium acetate 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 18.0h， 生成 (3S)-3-tert-butyldimethylsilyloxyandrost-5-en-17-one
  参考文献：
  名称：

  Identification and quantification of dehydroepiandrosterone sulphate in saliva

  摘要：

  3 beta-Hydroxy-5-androsten-17-one (dehydroepiandrosterone) sulphate has been separated from an extract of human saliva by ion exchange gel chromatography and identified by high resolution gas chromatography-high resolution mass spectrometry of the tert-butyldimethylsilyl derivative of the neutral steroid obtained by enzymic hydrolysis. Quantitative analyses, employing 7,7-2H-dehydroepiandrosterone sulphate as the internal standard, have indicated concentrations in the saliva of young adult subjects to be generally in the range 0.9-5.7 nmol/l, though concentrations as high as 10.7 nmol/l have been observed.

  DOI：

  10.1016/0039-128x(82)90126-x