albumin

• 分子结构分类: 水解酶 - 作用于肽键（肽酶） - 丝氨酸内肽酶
• 英文名称: albumin
• 英文别名: albumine；BSA

反应信息

• 作为试剂：
  描述：

  香叶醇 、 1,2-二油酰基-sn-甘油-3-磷酸胆碱 在 sodium acetate buffer 、 albumin 作用下， 以 乙酸乙酯 为溶剂， 反应 24.0h， 以90%的产率得到phosphatidylgeraniol
  参考文献：
  名称：

  通过磷脂酶D介导的转磷脂酰化制备磷脂酰化的萜烯

  摘要：

  AbstractTerpenes such as geraniol, geranylgeraniol, farnesol, and phytol are known as functional compounds which exhibit anticancer effects and activate nuclear receptors. For the application of functional terpenes in various fields, including the cosmetic and food industries, we attempted to synthesize phosphatidylated terpenes (terpene‐PLs) by using phospholipase D (PLD). Transphosphatidylation of phosphatidylcholine with terpenes was carried out using PLD in a biphasic system containing ethyl acetate/water or in an aqueous system without organic solvent. The yield of terpene‐PL increased with the reaction time and the amount of PLD in both the biphasic and aqueous systems. Further, the yield of terpene‐PL in the aqueous system was higher than that in the biphasic system. In addition, among four PLDs from Streptomyces sp., Streptomyces chromofuscus, cabbage, and peanut, only the PLD from Streptomyces sp. could synthesize terpene‐PL. The reaction yield, based on substrate phospholipid, of phosphatidylgeraniol reached 90 mol% under the following optimal reaction conditions: 50 μmol soyPC; 2,000 μmol geraniol; 1.6 U PLD; 0.8 ml of 0.2 M sodium acetate buffer (pH 5.6); temperature, 37 °C ; and reaction time, 24 h. The reaction yields of phosphatidylfarnesol, phosphatidylgeranylgeraniol, and phosphatidylphytol were 73, 54, and 17 mol%, respectively.

  DOI：

  10.1007/s11746-008-1206-1