galactosyldiacylglycerol alpha-2,3-sialyltransferase | 80237-98-5

• 分子结构分类: 转移酶 - 糖基转移酶 - 唾液酸转移酶
• 英文名称: galactosyldiacylglycerol alpha-2,3-sialyltransferase
• 英文别名: α-(2->6)-sialyltransferase
• CAS: 80237-98-5

反应信息

• 作为试剂：
  描述：

  2-脱氧-2-苯二酰亚胺-1,3,4,6-四-氧-乙酰-β-D-吡喃葡萄糖 在 galactosyldiacylglycerol alpha-2,3-sialyltransferase 、 三氟化硼乙醚 、 一水合肼 、 β-galactosidase 作用下， 以 甲醇 、 乙醇 、 二氯甲烷 为溶剂， 反应 57.0h， 生成
  参考文献：
  名称：

  β-Galactosidase-catalyzed intramolecular transglycosylation

  摘要：

  A novel transglycosylation strategy for efficient preparation of N-acetyllactosamine (Gal beta1 --> 4GlcNAc, LacNAc) and sialyl LacNAc was developed using beta -galactosidase from Bacillus circulans. In order to minimize the competing hydrolysis by forcing the enzymatic transglycosylation to proceed in an intramolecular manner, a novel substrate 9 carrying the donor (galactose) and the acceptor (N-acetylglucosamine) components linked via a 2-hydroxy-5-nitro-benzylalcohol derived tether was prepared. Treatment with beta -galactosidase from B. circulans afforded the transglycosylation product 11 in 26% yield. Furthermore, addition of sialyltransferase and CMP-sialic acid to this system gave sialyl LacNAc 18 in 39% yield. (C) 2001 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0040-4039(01)01826-3