NADH oxidase (H2O-forming)

• 分子结构分类: 氧化还原酶 - 作用于NADH或NADPH - 以氧为受体
• 英文名称: NADH oxidase (H2O-forming)
• 英文别名: H2O-forming NADH oxidase；Nox-2；recombinant Lactococcus lactis NADH oxidase

反应信息

• 作为试剂：
  描述：

  还原型辅酶Ⅰ 在 NADH oxidase (H2O-forming) HEPES buffer 、 氧气 、 腺嘌呤黄素 作用下， 以 水 为溶剂， 生成 nicotinamide adenine dinucleotide
  参考文献：
  名称：

  Hydrogen peroxide-producing NADH oxidase (nox-1) from Lactococcus lactis

  摘要：

  We have successfully applied the sequence comparison-based approach to develop a novel hydrogen peroxide-forming NADH oxidase (nox-1) from Lactococcus lactis (L. lactis) that reduces oxygen to hydrogen peroxide. The nox-1 gene (AhpF) was isolated from genomic L. lactis DNA by PCR and cloned into the expression vector pET32. The His-tagged protein was overexpressed at 20 degreesC after induction with 167 muM IPTG, and purified by Co2+-IMAC. After purification, nox-1 was found to be an apo-protein, so we reconstituted the holo-flavoenzyme with FAD cofactor, finding a 1:1 stoichiometry of FAD and nox-1 subunit and a Km value of 54 muM. The maximum specific activity of 15U/mg protein compares favorably to other nox-1 enzymes in the literature. While both products, NAD(+) and H2O2, inhibit nox-1, the enzyme seems rather robust in presence of moderate H2O2 concentrations. Titration of H2O2 formed with Amplex Red demonstrates that only half of the electrons from NADH go to H2O2, (C) 2004 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.tetasy.2004.07.057