antibody 23E₁₀

• 英文名称: antibody 23E₁₀

反应信息

• 作为试剂：
  描述：

  5-硝基-3-羧基苯并异恶唑 在 antibody 23E₁₀ 三羟甲基氨基甲烷盐酸盐 、 sodium chloride 作用下， 生成 2-羟基-5-硝基苯甲腈
  参考文献：
  名称：

  Catalysis of 3-Carboxy-1,2-benzisoxazole Decarboxylation by Hydrophobic Antibody Binding Pockets

  摘要：

  Monoclonal antibodies were generated against a 3-phenyl-1,2-benzisoxazole derivative and shown to catalyze the solvent-sensitive decarboxylation of 3-carboxy-1,2-benzisoxazoles. In addition to rare accelerations up to 2300-fold over background, the antibodies exhibit distinctive selectivities for substrates bearing 5- or 6-NO2 substituents, with preferential decarboxylation of the less reactive substrate in one case. These effects are the likely consequence of substrate destabilization, achieved by forcing the carboxylate group into a relatively apolar binding pocket and stabilization of the charge-delocalized transition state through dispersive interactions. Comparison with a more active antibody decarboxylase previously raised against 2-acetamido-naphthalene-1,5-disulfonate suggests, however, that a judicious mix of polar and apolar interactions may ultimately be more effective for achieving high decarboxylase activity.

  DOI：

  10.1002/1522-2675(20000906)83:9<2183::aid-hlca2183>3.0.co;2-c