rat hepatic microsome

• 英文名称: rat hepatic microsome

反应信息

• 作为试剂：
  描述：

  睾酮 在 四氯乙烯 、 rat hepatic microsome 作用下， 生成 6,17-二羟基-6b,17b-雄甾-4-烯-3-酮
  参考文献：
  名称：

  Interaction of tetrachloroethylene with rat hepatic microsomal P450-dependent monooxygenases

  摘要：

  1. We have studied the effects of tetrachloroethylene (PCE) on the kinetics of the P450-dependent monooxygenases in rat liver microsomes.2. 7-Pentoxyresorufin O-depentylase (PROD) and 7-benzyloxyresorufin O-debenzylase (BROD) activities in phenobarbital (PB)-treated rat liver microsomes were substantially inhibited by PCE. The inhibition profiles were non-competitive for both enzyme activities; K-i's from Eadie-Hofsee plots were 0.16 and 0.29 mM for PROD and BROD respectively. In contrast, the enzyme activities in untreated, beta-naphthoflavone (BNF)-, isoniazid (ISN)and pregnenolone-16 alpha-carbonitrile (PCN)-induced microsomes were not affected by PCE.3. 7-Ethoxycoumarin O-deethylase (ECOD) activity in PB-induced microsomes was competitively inhibited by PCE, with a K-i that was lower than those of other microsomes.4. PCE inhibited 7-ethoxyresorufin O-deethylase (EROD) activities in some microsomes slightly. The K-i for PCE was the lowest in untreated, followed by ISN-treated microsomes.5. No effect of PCE upon aniline 4-hydroxylase (AN4H) and testosterone 6 beta-hydroxylase (TS6BH) activities was evident in any microsomal preparation.6. These results indicate that PCE inhibits PB-inducible, P450-dependent monooxygenases in vitro non-competitively or competitively, and that the P450 enzymes of the P4502B subfamily may contribute to PCE toxicity.

  DOI：

  10.3109/00498259509061841