N_.meningitidis CMP-sialic acid synthetase

• 英文名称: N_.meningitidis CMP-sialic acid synthetase

反应信息

• 作为试剂：
  描述：

  N-acetyl-D-mannosamine 、 sodium pyruvate 、 4-甲基伞形-β-D-乳吡喃糖苷 在 E_.coli K₁₂ sialic acid aldolase 、 N_.meningitidis CMP-sialic acid synthetase 、 Tris-HCl buffer 、 DL-dithiothreitol DL-dithiothreitol 、 胞苷-5’-三磷酸 、 magnesium chloride 、 tPm₀₁₈₈Ph 作用下， 以 various solvent(s) 为溶剂， 反应 2.0h， 以5.5%的产率得到4-methylumbelliferyl O-(5-acetamido-3,5-dideoxy-D-glycero-α-D-galacto-2-nonulopyranosylonic acid)-(2->6)-O-β-D-galactopyranosyl-(1->4)-β-D-glucopyranoside
  参考文献：
  名称：

  A Multifunctional Pasteurella multocida Sialyltransferase:  A Powerful Tool for the Synthesis of Sialoside Libraries

  摘要：

  A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein is (1) an alpha2,3-sialyltransferase (main function), (2) an alpha2,6-sialyltransferase, (3) an alpha2,3-sialidase, and (4) an alpha2,3-trans-sialidase. The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality.

  DOI：

  10.1021/ja0561690