N_.meningitidis CMP-Sia synthetase

• 英文名称: N_.meningitidis CMP-Sia synthetase

反应信息

• 作为试剂：
  描述：

  羟甲香豆素 在 E_.coli K₁₂ sialic acid aldolase 、 N_.meningitidis CMP-Sia synthetase 4 A molecular sieve 、 tPm₀₁₈₈Ph 、 三氟化硼乙醚 、 sodium methylate 、 胞苷-5’-三磷酸 、 magnesium chloride 作用下， 以 甲醇 、 二氯甲烷 、 various solvents 为溶剂， 反应 12.0h， 生成 4-methylumbelliferyl O-(5-[(2-propynyloxy)carbonyl]amido-3,5-dideoxy-D-glycero-α-D-galacto-2-nonulopyranosylonic acid)-(2->3)-O-β-D-galactopyranosyl-(1->4)-β-D-glucopyranoside
  参考文献：
  名称：

  A Multifunctional Pasteurella multocida Sialyltransferase:  A Powerful Tool for the Synthesis of Sialoside Libraries

  摘要：

  A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein is (1) an alpha2,3-sialyltransferase (main function), (2) an alpha2,6-sialyltransferase, (3) an alpha2,3-sialidase, and (4) an alpha2,3-trans-sialidase. The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality.

  DOI：

  10.1021/ja0561690