YDR₃₆₈w GST-fusion protein from Saccharomyces cerevisiae

• 英文名称: YDR₃₆₈w GST-fusion protein from Saccharomyces cerevisiae

反应信息

• 作为试剂：
  描述：

  2-氯-3-氧代戊酸乙酯 在 KP_i 、 YDR₃₆₈w GST-fusion protein from Saccharomyces cerevisiae D-葡萄糖-6-磷酸 、 烟酰胺腺嘌呤双核苷酸磷酸盐 、 glucose 6-phosphate dehydrogenase 作用下， 生成 (2R,3S)-2-Chloro-3-hydroxy-pentanoic acid ethyl ester
  参考文献：
  名称：

  Stereoselective, Biocatalytic Reductions of α-Chloro-β-keto Esters

  摘要：

  Eighteen known and putative reductases from baker's yeast (Saccharomyces cerevisiae) were tested for the ability to reduce a series of alpha-chloro-beta-keto esters. In nearly all cases, it was possible to produce at least two of the four possible alpha-chloro-beta-hydroxy ester diastereomers with high optical purities. The utility of this approach was demonstrated by reducing ethyl 2-chloroacetoacetate to the corresponding Syn-(2R,3S)-alcohol on a multigram scale using whole cells of an Escherichia coli strain overexpressing a single yeast reductase identified from the screening studies.

  DOI：

  10.1021/jo0484981