wild-type epoxide hydrolase (Agrobacterium radiobacter AD₁)

• 英文名称: wild-type epoxide hydrolase (Agrobacterium radiobacter AD₁)

反应信息

• 作为试剂：
  描述：

  (S)-2-(4-硝基苯基)环氧乙烷 在 wild-type epoxide hydrolase (Agrobacterium radiobacter AD₁) tris-buffer 、 水 作用下， 以 二甲基亚砜 为溶剂， 生成 (S)-(+)-1-(4-nitrophenyl)-1,2-ethanediol
  参考文献：
  名称：

  Biocatalytic Potential of the Epoxide Hydrolase from Agrobacterium radiobacter AD1 and a Mutant with Enhanced Enantioselectivity

  摘要：

  Optically pure epoxides are useful synthons for a variety of biologically active compounds. The epoxide hydrolase obtained from Agrobacterium radiobacter AD1 hydrolyses racemic aryl epoxides with moderate and aliphatic epoxides with low enantioselectivity. The three-dimensional structure of this enzyme indicates that two tyrosine residues interact with the epoxide oxygen. Mutating one of these, tyrosine 215, to a phenylalanine (Y215F) resulted in an enzyme with increased enantioselectivity towards aryl epoxides. The relatively strong decrease in activity towards the remaining enantiomers makes this enzyme a much better biocatalyst than the wild-type enzyme for the preparation of optically pure (S)-styrene oxide derivatives.

  DOI：

  10.1002/1615-4169(200210)344:9<980::aid-adsc980>3.0.co;2-a