human serum albumin

• 英文名称: human serum albumin
• 英文别名: human serum albumine；HSA；018K7540；A1887；A8763；HAS

反应信息

• 作为反应物：
  描述：

  human serum albumin 生成 jatrorrhizine
  参考文献：
  名称：

  Spectroscopic analysis and molecular modeling on the interaction of jatrorrhizine with human serum albumin (HSA)

  摘要：

  In this work, the interaction of jatrorrhizine with human serum albumin (HSA) was studied by means of UV-vis and fluorescence spectra. The intrinsic fluorescence of HSA was quenched by jatrorrhizine, which was rationalized in terms of the static quenching mechanism. The results show that jatrorrhizine can obviously bind to HSA molecules. According to fluorescence quenching calculations, the bimolecular quenching constant (k(q)), apparent quenching constant (K-SV) at different temperatures were obtained. The binding constants K are 4059 L mol(-1) and 1438 L mol(-1) at 299 K and 304 K respectively, and the number of binding sites n is almost 1. The thermodynamic parameters determined by the Van't Hoff analysis of the binding constants (Delta R -12.25 kJ mol(-1) and AS 28.17 J mol(-1) K-1) clearly indicate that the electrostatic force plays a major role in the process. The efficiency of energy transfer and the distance between the donor (HSA) and the acceptor (jatrorrhizine) were calculated as 22.2% and 3.19 nm according to Foster's non-radiative energy transfer theory. In addition, synchronous fluorescence spectroscopy reveals that jatrorrhizine can influence HSA's microstructure. That is, jatrorrhizine is more vicinal to tryptophane (Trp) residue than to tyrosine (Tyr) residue and the damage site is also mainly at Trp residue. Molecular modeling result shows that jatrorrhizine-HSA complex formed not only on the basis of electrostatic forces, but also on the basis of pi-pi staking and hydrogen bond. The research results will offer a reference for the studies on the biological effects and action mechanism of small molecule with protein. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

  DOI：

  10.1016/j.saa.2013.07.029
• 作为试剂：
  描述：

  1-甲基-3-对甲苯基三氮 在 phosphate buffer 、 human serum albumin 作用下， 生成 乙烷,三氯氟- 、 alkaline earth salt of/the/ methylsulfuric acid
  参考文献：
  名称：

  在近乎生理条件下，血清白蛋白对对位取代的1-苯基-3-甲基三氮烯和5-（3-甲基-1-三氮烯基）咪唑-4-羧酰胺的分解速率的影响。

  摘要：

  研究了人血清白蛋白对某些芳基单甲基三氮烯在缓冲水溶液中分解速率的影响。从实验数据中，得出了三氮烯-白蛋白相互作用的模型，并通过两种独立的方法系统地计算了热力学参数。结果表明，结合的能量对三氮烯芳族环中的取代基有明显的依赖性。对于大多数研究的三氮烯，与白蛋白的结合主要是焓驱动的。还使用牛和鼠血清白蛋白进行测量。

  DOI：

  10.1002/jps.2600700815