(2E,6E)-farnesyl diphosphate synthase | 37277-79-5

• 分子结构分类: 转移酶 - 转移烷基或芳基（甲基除外） - 转移除甲基以外的烷基或芳基（有子类）
• 英文名称: (2E,6E)-farnesyl diphosphate synthase
• 英文别名: farnesyl-diphosphate synthase；geranyl transferase I；prenyltransferase；farnesyl pyrophosphate synthetase；farnesylpyrophosphate synthetase；geranyltranstransferase；recombinant avian farnesyl diphosphate synthase
• CAS: 37277-79-5

反应信息

• 作为试剂：
  描述：

  二磷酸 P-(3-甲基-3-丁烯-1-基)酯 、 香叶基焦磷酸铵盐 在 (2E,6E)-farnesyl diphosphate synthase 5-降冰片烯-2,3-二羧酸 、 S-geranylgeranyl thiodiphosphate 、 2-巯基乙醇 作用下， 以 水 为溶剂， 反应 0.17h， 生成 farnesyl pyrophosphate
  参考文献：
  名称：

  Synthesis of (S)-Isoprenoid Thiodiphosphates as Substrates and Inhibitors

  摘要：

  Thiolo thiophosphate analogues of isopentenyl diphosphate (IPP), dimethylallyl. diphosphate (DMAPP), geranyl diphosphate (GPP), farnesyl diphosphate (FPP), and geranylgeranyl diphosphate (GGPP) were synthesized, Inorganic thiopyrophosphate (SPP) was prepared from trimethyl phosphate in four steps. The tris(tetra-n-butylammonium) salt was then used to convert isopentenyl tosylate to (S)-isopentenyl thiodiphosphate (ISPP). (S)-Dimethylallyl (DMASPP), (S)-geranyl (GSPP), (S)-farnesyl (FSPP), and (S)-geranylgeranyl thiodiphosphate (GGSPP) were prepared from the corresponding bromides in a similar manner. ISPP and GSPP were substrates for avian farnesyl diphosphate synthase (FPPase). Incubation of the enzyme with ISPP and GPP gave FSPP, whereas incubation with IPP and GSPP gave FPP. GSPP was a substantially less reactive than GPP in the chain elongation reaction and was an excellent competitive inhibitor, K-I(GSPP) = 24.8 muM, of the enzyme. Thus, when ISPP and DMAPP were incubated with FPPase, GSPP accumulated and was only slowly converted to FSPP.

  DOI：

  10.1021/jo010505n