human milk fucosyltransferase

• 英文名称: human milk fucosyltransferase

反应信息

• 作为试剂：
  描述：

  bis(ammonium) guanosine 5'-(β-L-fucopyranosyl) diphosphate 、 2-巯基乙醇 、 2-(3'-mercaptopropionylamido)ethyl β-D-galactopyranosyl-(1->3)-O-2-acetamido-2-deoxy-β-D-glucopyranoside 在 human milk fucosyltransferase Thiopyridyl-activated Thiopropyl Sepharose 6B gel 、 sodium azide 、 sodium cacodylate 、 manganese(ll) chloride 作用下， 以 水 、 甘油 为溶剂， 反应 145.0h， 以68%的产率得到
  参考文献：
  名称：

  Solid-Phase Enzymatic Synthesis of a Lewis a Trisaccharide Using an Acceptor Reversibly Bound to Sepharose

  摘要：

  The disaccharide 2-aminoethyl O-beta-D-galactopyranosyl-(1-->3)-2-acetamido-2-deoxy-beta-D-glucopyranoside was reacted with thiobutyrolactone to give a disaccharide with a thiol group on the aglycone. This disaccharide was reacted with activated Thiopropyl Sepharose, which gave a disaccharide bound to Sepharose via a disulphide bond. Enzymatic fucosylation, using GDP-fucose and partially purified human milk fucosyltransferase, gave a trisaccharide in good yield, which was cleaved from Sepharose by treatment with mercaptoethanol or dithiothreitol.

  DOI：

  10.1080/07328309708006516