choloyl-CoA hydrolase | 37270-64-7

• 分子结构分类: 水解酶 - 作用于酯键 - 硫酯水解酶
• 英文名称: choloyl-CoA hydrolase
• 英文别名: PTE-2 (ambiguous)；choloyl-coenzyme A thioesterase；chenodeoxycholoyl-coenzyme A thioesterase；peroxisomal acyl-CoA thioesterase 2；peroxisomal nafenopin-CoA ligase
• CAS: 37270-64-7；9025-87-0

反应信息

• 作为试剂：
  描述：

  萘酚平 、 辅酶 A 在 choloyl-CoA hydrolase 、 magnesium chloride 作用下， 反应 4.0h， 生成 萘酚平-辅酶A
  参考文献：
  名称：

  Kinetic characteristics of rat liver peroxisomal nafenopin-CoA ligase

  摘要：

  In this study we have demonstrated that rat hepatic peroxisomes catalyse the formation of nafenopin-CoA The process is mediated by apparent high affinity (K-m 6.7 mu M), low capacity (V-max 0.31 nmol/mg/min) and low affinity, high capacity isoforms. Palmitic acid (K-i 1.1 mu M), R(-) ibuprofen (K-i 7.9 mu M), ciprofibrate (K-i 60.2 mu M) and clofibric acid (K-i 86.8 mu M) competitively inhibited nafenopin-CoA formation catalysed by the apparent high affinity isoform. An antibody raised against the microsomal palmitoyl-CoA ligase inhibited the equivalent peroxisomal enzyme significantly (P < 0.001) but did not inhibit peroxisomal nafenopin-CoA ligase activity. These data suggest that nafenopin-CoA formation is catalysed by a peroxisomal CoA ligase which differs from the peroxisomal long chain fatty acid-CoA ligase in relation to its xenobiotic/antibody inhibitor profile and kinetic characteristics.

  DOI：

  10.1016/0006-2952(94)00516-o