dethiobiotin synthase | 37259-75-9

• 分子结构分类: 连接酶 - 形成碳氮键 - 环连接酶
• 英文名称: dethiobiotin synthase
• 英文别名: desthiobiotin synthase；dethiobiotin synthetase of E_. coli
• CAS: 37259-75-9

反应信息

• 作为试剂：
  描述：

  碳酸氢钠 、 (7R,8S)-7,8-diaminononanoic acid 在 dethiobiotin synthase 、 E_.C_. 6.6.6.6 、 二叔丁基硅烷 5’-三磷酸腺苷 作用下， 以 水 为溶剂， 生成 D-脱硫生物素
  参考文献：
  名称：

  The design and synthesis of inhibitors of dethiobiotin synthetase as potential herbicides

  摘要：

  Dethiobiotin synthetase (DTBS;E.C. 6.6.6.6), the penultimate enzyme in the biosynthesis of the essential vitamin biotin, is a new potential target for novel herbicides. Inhibitors were designed based on mechanistic and structural information. The in-vitro activities of these potential inhibitors versus the bacterial enzyme are reported here. Mimics of 7,8-diaminopelargonic acid (DAPA) or the DAPA carbamate reaction intermediate were substrates or partial substrates for the enzyme. Synergistic binding with ATP was noted with compounds which contained an amino functionality, NMR studies and X-ray structures confirmed that the inhibitors could be phosphorylated by the enzyme. Several series of potential inhibitors were designed to take advantage of this partial substrate activity by generating potentially more tightly bound phosphorylated inhibitors in situ, Structure-activity relationships for these series based on both substrate and inhibitory activity are described herein. An X-ray structure for one of these inhibitors is also discussed. Although considerable potential for inhibitors of this type was demonstrated, none of the compounds reported showed sufficient herbicidal activity to be a commercial proposition. (C) 1999 Society of Chemical Industry.

  DOI：

  10.1002/(sici)1096-9063(199903)55:3<236::aid-ps888>3.0.co;2-0