4,4-difluoro-8-(2-(2-(aminooxy)acetamido)phenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene | 1301265-83-7

• 英文名称: 4,4-difluoro-8-(2-(2-(aminooxy)acetamido)phenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene
• CAS: 1301265-83-7
• 化学式: C₂₁H₂₃BF₂N₄O₂
• 分子量: 412.247
• InChiKey: RHTDSWYPJXRGQB-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
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• 重原子数：
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• 可旋转键数：
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• 环数：
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• sp3杂化的碳原子比例：
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• 拓扑面积：
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• 氢给体数：
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• 氢受体数：
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反应信息

• 作为产物：
  描述：

  4,4-difluoro-8-(2-(2-((1,3-dioxoisoindolin-2-yl)oxy)acetamido)phenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene 在 一水合肼 作用下， 以 四氢呋喃 、 甲醇 为溶剂， 反应 20.0h， 以70%的产率得到4,4-difluoro-8-(2-(2-(aminooxy)acetamido)phenyl)-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene
  参考文献：
  名称：

  Live Cell Labeling of Native Intracellular Bacterial Receptors Using Aniline-Catalyzed Oxime Ligation

  摘要：

  Live cell fluorescent labeling of proteins has become a seminal tool in biology and has led to hallmark discoveries in diverse research areas such as protein trafficking, cell-to-cell interactions, and intracellular network dynamics. One of the main challenges, however, remains the ability to label intracellular proteins using fluorescent ligands with high specificity, all the while retaining viability of the targeted cells. Here, we present the first example of live cell labeling and imaging of an intracellular bacterial receptor involved in cell-to-cell communication (i.e., quorum sensing), using a novel two-step approach involving covalent attachment of a reactive mimic of the primary endogenous Pseudomonas aeruginosa quorum-sensing signal to its receptor, LasR, followed by aniline-catalyzed mime formation between the modified receptor and a fluorescent BODIPY derivative. Our results indicate that LasR is not distributed evenly throughout the cytoplasmic membrane but is instead concentrated at the poles of the P. aeruginosa cell.

  DOI：

  10.1021/ja200455d