emodin deoxygenase

• 英文名称: emodin deoxygenase

反应信息

• 作为试剂：
  描述：

  emodin 在 emodin deoxygenase 、 还原型辅酶II(NADPH)四钠盐 作用下， 生成 大黄酚
  参考文献：
  名称：

  NADPD isotope effect on activity of emodin deoxygenase

  摘要：

  Thermoanaerobium brokii alcohol dehydrogenase catalysed the transfer of the pro 4H(s) hydrogen of NADPH. The effect of 4-deuteration of NADPH on the initial rate of conversion of emodin to chrysophanol catalysed by emodin deoxygenase was determined. The deuterium isotope effects on V/K(m) and V(max) with [4R-H-2] NADPH were 1.20 +/- 0.36 and 1.10 +/- 0.11, respectively, The deuterium isotope effects on V/K(m) and V(max) with [4S-H-2]NADPH were 4.08 +/- 1.55 and 6.90 +/- 0.74, respectively. Emodin deoxygenase, therefore, transfers the 4S hydrogen of NADPD in the rate-determining step.

  DOI：

  10.1016/0031-9422(93)85210-i