rabbit liver microsomes

• 英文名称: rabbit liver microsomes

反应信息

• 作为试剂：
  描述：

  盐酸莱克多巴胺 、 6-[[[5-(2,4-二氧代嘧啶-1-基)-3,4-二羟基四氢呋喃-2-基]甲氧基-羟基磷酰]氧基-羟基磷酰]氧基-3,4,5-三羟基四氢吡喃-2-羧酸 在 rabbit liver microsomes 作用下， 以 phosphate buffer 为溶剂， 生成 ractopamine 10'-glucuronide 、 ractopamine 10-glucuronide
  参考文献：
  名称：

  Identification of turkey biliary metabolites of ractopamine hydrochloride and the metabolism and distribution of synthetic [¹⁴C]ractopamine glucuronides in the turkey

  摘要：

  1. The bile duct cannulated turkey poult (n = 3) dosed orally with [C-14]ractopamine HCl {(1R*, 3R*),(1R*,3S*)-4-hydroxy-alpha-[[[3-(4-hydroxy[C-14]phenyl)-1-methylpropyl]- amino]methyl]-benzenemethanol hydrochloride; 19.9 mg; 9.28 mu Ci} excreted 37.4 +/- 12.1% (mean+/-SD) of the administered radioactivity in bile by 24 h post-dosing.2. A mono-glucuronide, conjugated at C-10 (the methylpropylamino phenol) of ractopamine, accounted for 76.6 % of biliary radioactivity.3. Urine collected from the colostomized turkey poult (n = 3) orally dosed with synthetic [C-14]ractopamine-glucuronides (10.1 mg; 3.6 mu Ci) contained 11.9+/-1.0% (mean+/-SD) of the administered radioactivity 24 h after dosing. indicating that some absorption of radioactivity occurred. Faeces contained 60.6% of the administered radioactivity and carcasses (with gastrointestinal tracts) contained 23.3 %, of the starting radioactivity.4. Five colostomized poults were fitted with bile duct cannulas and were dosed intraduodenally with 10.2 mg (3.6 mu Ci) synthetic [C-14]ractopamine-glucuronides. Urine and bile contained 15.5 +/- 2.2 and 16.8 +/- 2.1 % respectively of the administered radiocarbon by 24 h post-dosing. Faeces contained 54.3% of the administered radioactivity. Total absorption of the dosed radioactivity aleraged 33.4 %.5. Bile and urine collected from the colostomized, bile-duct cannulated bird contained mainly ractopamine glucuronides. Indirect evidence suggests that the dosed ractopamine glucuronides were nor absorbed intact.

  DOI：

  10.1080/004982500237613