enzyme system adrenodoxin

• 分子结构分类: 氧化还原酶 - 作为供体作用于铁硫蛋白
• 英文名称: enzyme system adrenodoxin

反应信息

• 作为试剂：
  描述：

  胆固醇 在 adrenodoxin reductase 、 cytochrome P-450scc 、 enzyme system adrenodoxin 作用下， 生成 孕烯醇酮
  参考文献：
  名称：

  3-methoxybenzidine: A potent inhibitor of cholesterol side chain cleavage cytochrome P-450

  摘要：

  The effect of 3-methoxybenzidine on the conversion of cholesterol to pregnenolone was investigated using a reconstituted enzyme system comprised of adrenodoxin, adrenodoxin reductase and cytochrome P-450scc purified from bovine adrenal cortex. Under conditions where the cytochrome P-450scc concentration was rate-limiting, 3-methoxybenzidine was found to be a potent inhibitor, causing 50% inhibition at 7 microM when using a cholesterol concentration of 70 microM. The parent compound, benzidine, was much less effective, exhibiting an I50 value of approximately 40 microM. No effect of 3-methoxybenzidine was observed on the adrenodoxin reductase and adrenodoxin-catalyzed reduction of cytochrome c by NADPH, and it is concluded that 3-methoxybenzidine acts on cytochrome P-450scc in inhibiting cholesterol side chain cleavage.

  DOI：

  10.1016/0039-128x(80)90034-3