endopeptidase Ia from jojoba seeds

• 英文名称: endopeptidase Ia from jojoba seeds

反应信息

• 作为试剂：
  描述：

  Bz Arg-p-nitroanilide 在 endopeptidase Ia from jojoba seeds 水 作用下， 反应 1.0h， 生成 4-硝基苯胺
  参考文献：
  名称：

  Multiple forms of endopeptidase activity from Jojoba seeds

  摘要：

  The cotyledons of 27 day post-germination jojoba seedlings (Simmondsia chinensis) contained five distinct endopeptidase activities separable by DEAE Bio-Gel and CM-cellulose ion exchange chromatography. The endopeptidases were purified 108- to 266-fold and their individuality was confirmed by activity-specific assays in native acrylamide gels along with differences in their Mr and catalytic properties. The five endopeptidases, which showed activity on model substrates and protein, were named EP Ia, EP Ib, EP II, EP III and EP IV. EP Ia was a serine proteinase with a pH optimum of ca 8 and Mr of 58,000. EP Ib, II and III were discrete cysteine proteinases showing pH optima of ca 6.8, 6.0 and 5.4 and Mr of 41,000, 47,000 and 35,000 respectively. EP IV was an aspartic acid proteinase with a ca pH optimum of 3.5 and Mr of 33,000.

  DOI：

  10.1016/0031-9422(90)85159-d