Escherichia coli BL₂₁(DE₃)(pIK₃₀) cells

• 英文名称: Escherichia coli BL₂₁(DE₃)(pIK₃₀) cells

反应信息

• 作为试剂：
  描述：

  2-氯乙酰乙酸乙酯 在 phosphate buffer 、 Escherichia coli BL₂₁(DE₃)(pIK₃₀) cells 氢氧化钾 、 air 、 葡萄糖 作用下， 反应 8.0h， 以89%的产率得到ethyl 2-chloro-3-hydroxybutyrate
  参考文献：
  名称：

  Stereoselective, Biocatalytic Reductions of α-Chloro-β-keto Esters

  摘要：

  Eighteen known and putative reductases from baker's yeast (Saccharomyces cerevisiae) were tested for the ability to reduce a series of alpha-chloro-beta-keto esters. In nearly all cases, it was possible to produce at least two of the four possible alpha-chloro-beta-hydroxy ester diastereomers with high optical purities. The utility of this approach was demonstrated by reducing ethyl 2-chloroacetoacetate to the corresponding Syn-(2R,3S)-alcohol on a multigram scale using whole cells of an Escherichia coli strain overexpressing a single yeast reductase identified from the screening studies.

  DOI：

  10.1021/jo0484981