Helix pomatia albumen gland sediment

• 英文名称: Helix pomatia albumen gland sediment

反应信息

• 作为试剂：
  描述：

  β-D-Galp-(1->3)-β-D-Galp-O-CH3 、 5`-二磷酸鸟嘌呤核苷-岩藻糖二钠盐 在 Helix pomatia albumen gland sediment sodium azide 、 calf intestine alkaline phosphatase EC 3.1.3.1 、 manganese(ll) chloride 作用下， 以 various solvent(s) 为溶剂， 反应 18.0h， 以93%的产率得到Fuc(a1-2)Gal(b1-3)b-Gal1Me
  参考文献：
  名称：

  Enzymatic α(1→2)-l-fucosylation: investigation of the specificity of the α(1→2)-l-galactosyltransferase from Helix pomatia

  摘要：

  The alpha(1-->2)-L-galactosyltransferase from Helix pomatia transfers an L-fucosyl residue from GDP-L-Fucose to a terminal, non-reducin D-galactopyranosyl moiety of an oligosaccharide. The extent of the enzyme's specificity towards the stereochemistry at the D-galactopyranosyl anomeric centre, the site of interglycosidic linkage and the nature of the subterminal oliaosaccharide residue has been investigated using HPAEC-PAD and MALDI-TOF technology. This alpha(1-->2)-L-galactosyltransferase is specific for D-galactopyranosyl beta-linkages, independent of the site of the interglycosidic linkage and aglycone configuration and with limited specificity for the nature of the subterminal sugar residue. (C) 2003 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/s0957-4166(03)00521-4