E_. coli D-1-deoxyxylulose-5-phosphate reductoisomerase

• 英文名称: E_. coli D-1-deoxyxylulose-5-phosphate reductoisomerase

反应信息

• 作为试剂：
  描述：

  DXP 钠盐 在 magnesium chloride E_. coli D-1-deoxyxylulose-5-phosphate reductoisomerase 、 三羟甲基氨基甲烷盐酸盐 、 还原型辅酶II(NADPH)四钠盐 作用下， 以 水 为溶剂， 生成 (2R,3S)-2,3,4-三羟基-3-甲基丁基磷酸二氢酯
  参考文献：
  名称：

  Molecular cloning, expression and characterization of the first three genes in the mevalonate-independent isoprenoid pathway in Streptomyces coelicolor

  摘要：

  The mevalonate-independent biosynthetic pathway to isopentenyl diphosphate and dimethylallyl diphosphate, the universal precursors to the isoprenoids, operates in eubacteria, including Escherichia coli, in algae, and in the plastids of higher plants. A search of the Sanger Centre Streptomyces coelicolor genome database revealed open reading frames with ca. 40-50% identity at the deduced amino acid level to the first three E. coli enzymes of this pathway, corresponding to deoxyxylulose phosphate synthase, deoxyxylulose phosphate reductoisomerase and 2-C-methyl erythritol 4-phosphate cytidylyltransferase. The S. coelicolor genes have been cloned and expressed in E. coli, and the recombinant proteins characterized physically and kinetically. The presence of the corresponding enzyme activities in extracts of S. coelicolor CH999 further supports the operation of the mevalonate-independent pathway in this organism. (C) 2001 Elsevier Science Ltd. All rights reserved.

  DOI：

  10.1016/s0968-0896(01)00050-5