| 1416050-22-0

• CAS: 1416050-22-0
• 化学式: C₁₆H₁₅O₃W*Cl
• 分子量: 474.596
• InChiKey: CDOMAFHHXGZQAU-UHFFFAOYSA-L

反应信息

• 作为产物：
  描述：

  麦芽醇 、 bis(cyclopentadienyl)tungsten dichloride 在 sodium methylate 作用下， 以 甲醇 为溶剂， 以42 %的产率得到
  参考文献：
  名称：

  具有多种 (O,O–)、(O,S–) 和 (O,N–) 螯合物的抗癌茂钨——通过 3D 球体模型进行改进评估的详细生物学研究

  摘要：

  描述了具有不同生物活性 (O,O–)、(S,O–) 和 (N,O–) 螯合物的茂钨的合成、表征和生物活性。通过 1H 和 13C NMR、元素分析、ESI 质谱、FT-IR 光谱和 X 射线衍射分析对配合物进行了表征。通过紫外/可见光谱研究水稳定性，通过循环伏安法研究 WIV 到 WV 过程。通过 MTT 法测定 A549、CH1/PA-1 和 SW480 癌细胞以及 IMR-90 人成纤维细胞中的细胞毒性。在单层和多细胞肿瘤球培养物中的其他三种人类癌细胞系（HCT116、HT29 和 MCF-7）中进行了广泛的生物学评估，以更好地了解作用模式。先导化合物在所有癌细胞系中都显示出有前景的体外抗癌活性。进一步的研究对细胞凋亡诱导、ROS 生成、金属分布的不同模式（通过 LA-ICP-TOF-MS 检测）、KI67（增殖标记）表达和 DNA 相互作用的变化产生了重要见解。基于定性和定量研究设计的结果表明，含有

  DOI：

  10.3390/pharmaceutics15071875

文献信息

• New tungstenocenes containing 3-hydroxy-4-pyrone ligands: antiproliferative activity on HT-29 and MCF-7 cell lines and binding to human serum albumin studied by fluorescence spectroscopy and molecular modeling methods
  作者：Moralba Domínguez-García、Carlos Ortega-Zúñiga、Enrique Meléndez

  DOI：10.1007/s00775-012-0964-2

  日期：2013.2

  Three new water-soluble tungstenocene derivatives were synthesized and characterized using 3-hydroxy-4-pyrone ligands, which provide aqueous stability to the complexes. The antiproliferative activities of the complexes on HT-29 colon cancer and MCF-7 breast cancer cell lines were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and showed the new tungstenocene derivatives have higher antiproliferative action than tungstenocene dichloride (Cp2WCl2, where Cp is cyclopentadienyl). The binding interactions of the tungstenocenes with human serum albumin (HSA) were investigated using fluorescence spectroscopy and molecular modeling methods. Analysis of the fluorescence quenching spectra indicates that the tungstenocene complexes bind HSA by hydrophobic interactions and hydrogen bonding at fatty acid binding site 6 and drug binding site II. Docking studies provided a description of the hydrophobic interactions and hydrogen bonding by which the tungstenocenes become engaged with HSA. It was determined that the binding affinity of the tungstenoecenes for HSA is in the order Cp2WCl2 < [Cp2W(ethyl maltolato)]Cl < [Cp2W(maltolato)]Cl < [Cp2W(kojato)]Cl, consistent with the hydrophobic interactions and the number of hydrogen bonds involved.