dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase

• 分子结构分类: 转移酶 - 转移含氮基团 - 转氨酶
• 英文名称: dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase
• 英文别名: SpnR；TDP-4-keto-2,3,6-trideoxy-D-glucose 4-aminotransferase；spnR from spinosyn gene cluster of Saccharopolyspora spinosa

反应信息

• 作为试剂：
  描述：

  在 phosphate buffer dTDP-4-dehydro-2,3,6-trideoxy-D-glucose 4-aminotransferase 、 glutamate 作用下， 生成
  参考文献：
  名称：

  Characterization of Protein Encoded by spnR from the Spinosyn Gene Cluster of Saccharopolyspora spinosa:  Mechanistic Implications for Forosamine Biosynthesis

  摘要：

  d-Forosamine is a 4-N,N-(dimethylamino)-2,3,4,6-tetradeoxy-alpha-d-threo-hexopyranose found in spinosyn produced by Saccharopolyspora spinosa. Studies of spinosyn biosynthesis in S. spinosa led to the isolation of the entire biosynthetic gene cluster. Heterologous expression of spnR, one putative gene in forosamine biosynthesis, in E. coli and purification of the SpnR protein identified it as an aminotransferase catalyzing the conversion of the 4-keto-2,3,6-trideoxy sugar intermediate to the corresponding 4-amino sugar product. Identification of SpnR function relied on the use of a stable TMP-phosphonate sugar in place of TDP-sugar substrate to determine the function of SpnR. This strategy may find general applicability for designing probes to study enzymes which catalyze the transformation of labile deoxysugar intermediates.

  DOI：

  10.1021/ja042702k