(R)-2-methylpyrrolidinium

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 吡咯烷类
• 英文名称: (R)-2-methylpyrrolidinium
• 英文别名: (2R)-2-methylpyrrolidin-1-ium
• 化学式: C5H12N+
• 分子量: 86.16
• InChiKey: RGHPCLZJAFCTIK-RXMQYKEDSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  0.7
• 重原子数：
  6
• 可旋转键数：
  0
• 环数：
  1.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  16.6
• 氢给体数：
  1
• 氢受体数：
  0

反应信息

• 作为反应物：
  描述：

  (R)-2-methylpyrrolidinium 、 NADP⁺ 生成 2-Methyl-1-pyrrolinium 、 氢(+1)阳离子 、 NADPH(4-)
  参考文献：
  名称：

  SUPPRESSION OF GRAFT-VERSUS-HOST REACTIVITY BY A SINGLE HOST-SPECIFIC BLOOD TRANSFUSION TO PROSPECTIVE DONORS OF HEMOPOIETIC CELLS

  摘要：

  在致命辐照异体重建小鼠的移植物抗宿主反应早期，可能会出现针对受体组织相容性抗原的迟发型超敏反应。这种反应性可以通过向异体脾细胞潜在供体单次输注宿主特异性血液来抑制。在小鼠用于重建致命辐照宿主之前4或5天输血时，抑制效果最佳。全血和纯化的白细胞能够诱导抑制，而纯化的红细胞、血浆和血清则不能。输注1±l全血后即可检测到抑制，而1.0ml剂量时几乎完全抑制。辐照血液会降低但不会消除其诱导抑制的能力。纯化的B淋巴细胞和纯化的T淋巴细胞在诱导抑制方面同样有效。两种辅助性T细胞克隆（Thl和Th2克隆）也能诱导抑制。重建后每天注射5天高剂量重组IL-2不会消除或降低抑制。抑制可以由H-2和非H-2同种抗原单独或共同诱导。纯H-2不相容输血比纯非H-2不相容输血更有效地诱导抑制。抑制似乎是一种主导现象，由Thy-1+、CD4+、CD8∼脾细胞群介导。这种T细胞群起源于输注的供体，排除了血液来源的“否决细胞”的可能参与。

  DOI：

  10.1097/00007890-199109000-00030

文献信息

• A NADPH-dependent (S)-imine reductase (SIR) from Streptomyces sp. GF3546 for asymmetric synthesis of optically active amines: purification, characterization, gene cloning, and expression
  作者：Koichi Mitsukura、Tatsuya Kuramoto、Toyokazu Yoshida、Norihiro Kimoto、Hiroaki Yamamoto、Toru Nagasawa

  DOI：10.1007/s00253-012-4629-4

  日期：2013.9

  A NADPH-dependent (S)-imine reductase (SIR) was purified to be homogeneous from the cell-free extract of Streptomyces sp. GF3546. SIR appeared to be a homodimer protein with subunits of 30.5 kDa based on SDS-polyacrylamide gel electrophoresis and HPLC gel filtration. It also catalyzed the (S)-enantioselective reduction of not only 2-methyl-1-pyrroline (2-MPN) but also 1-methyl-3,4-dihydroisoquinoline and 6,7-dimethoxy-1-methyl-3,4-dihydroisoquinoline. Specific activities for their imines were 130, 44, and 2.6 nmol min(-1) mg(-1), and their optical purities were 92.7 % ee, 96.4 % ee, and > 99 % ee, respectively. Using a NADPH-regenerating system, 10 mM 2-MPN was converted to amine with 100 % conversion and 92 % ee after 24 h. The amino acid sequence analysis revealed that SIR showed about 60 % identity to 6-phosphogluconate dehydrogenase. However, it showed only 37 % identity with Streptomyces sp. GF3587 (R)-imine reductase. Expression of SIR in Escherichia coli was achieved, and specific activity of the cell-free extract was about two times higher than that of the cell-free extract of Streptomyces sp. GF3546.