7-carboxyfluorescein

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 英文名称: 7-carboxyfluorescein
• 英文别名: 3',6'-Dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-4-carboxylic acid
• 化学式: C₂₁H₁₂O₇
• 分子量: 376.322
• InChiKey: JHFJMXJABIZQKC-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.9
• 重原子数：
  28
• 可旋转键数：
  1
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.05
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  7

反应信息

• 作为反应物：
  描述：

  7-carboxyfluorescein 在 甲醇 、 三氟乙酸 、 sodium hydroxide 作用下， 以 四氢呋喃 、 1,4-二氧六环 、 二氯甲烷 为溶剂， 反应 9.33h， 生成 7-methoxycarbonylfluorescein ditriflate
  参考文献：
  名称：

  BRIGHT TARGETABLE RED CA2+ INDICATORS

  摘要：

  目前披露的主题包括荧光指示剂，包括明亮和可靶向的红色Ca2+指示剂。目前披露的主题还包括包含相同指示剂的试剂盒，以及使用这些试剂盒来检测目标物质的方法。

  公开号：

  US20210054001A1
• 作为产物：
  描述：

  1,2,3-苯三甲酸 、 间苯二酚 在 甲烷磺酸 作用下， 反应 0.5h， 生成 7-carboxyfluorescein
  参考文献：
  名称：

  BRIGHT TARGETABLE RED CA2+ INDICATORS

  摘要：

  目前披露的主题包括荧光指示剂，包括明亮和可靶向的红色Ca2+指示剂。目前披露的主题还包括包含相同指示剂的试剂盒，以及使用这些试剂盒来检测目标物质的方法。

  公开号：

  US20210054001A1

文献信息

• BRIGHT TARGETABLE RED CA2+ INDICATORS
  申请人：HOWARD HUGHES MEDICAL INSTITUTE

  公开号：US20210054001A1

  公开（公告）日：2021-02-25

  The presently-disclosed subject matter includes fluorescent indicators, including bright and targetable red Ca2+ indicators. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.

  目前披露的主题包括荧光指示剂，包括明亮和可靶向的红色Ca2+指示剂。目前披露的主题还包括包含相同指示剂的试剂盒，以及使用这些试剂盒来检测目标物质的方法。
• Graebe; Leonhardt, Justus Liebigs Annalen der Chemie, 1896, vol. 290, p. 232
  作者：Graebe、Leonhardt

  DOI：——

  日期：——
• REPROGRAMMING UROKINASE INTO AN ANTIBODY-RECRUITING ANTICANCER AGENT
  申请人：YALE UNIVERSITY

  公开号：US20140308342A1

  公开（公告）日：2014-10-16

  The present invention relates to chimeric (preferably, bifunctional) compounds, compositions comprising those compounds and methods of treating cancer in a patient or subject, especially including metastatic cancer where cancer cells exhibit overexpression (heightened expression) of cell surface urokinase-type plasminogen activator receptor (urokinase receptor) compared to normal (non-cancerous) cells. The compounds preferably covalently bind to the urokinase receptor and recruit native antibodies of the patient or subject where the antibodies can selectively degrade and/or deactivate targeted cancer cells through antibody-dependent cellular phagocytosis and/or antibody-dependent cellular cytotoxicity (ADCC) against a large number and variety of cancers, thus providing cancer cell death and/or an inhibition of growth, elaboration and/or metastasis of the cancer, including remission and cure of the patient's cancer.
• [EN] DEVICES AND METHODS FOR CONCENTRATION AND ANALYSIS OF FLUIDS<br/>[FR] DISPOSITIFS ET PROCÉDÉS UTILISABLES EN VUE DE LA CONCENTRATION ET DE L'ANALYSE DE FLUIDES
  申请人：HALUSHKA PERRY V

  公开号：WO2012078308A1

  公开（公告）日：2012-06-14

  Disclosed are articles, compositions and methods for detecting analytes. The disclosed articled, compositions and methods increase the ease of detection and quantitation of the target analyte.
• Isomeric Tuning Yields Bright and Targetable Red Ca²⁺ Indicators
  作者：Claire Deo、Shu-Hsien Sheu、Jinyoung Seo、David E. Clapham、Luke D. Lavis

  DOI：10.1021/jacs.9b06092

  日期：2019.9.4

  Targeting small-molecule fluorescent indicators using genetically encoded protein tags yields new hybrid sensors for biological imaging. Optimization of such systems requires redesign of the synthetic indicator to allow cell-specific targeting without compromising the photophysical properties or cellular performance of the small-molecule probe. We developed a bright and sensitive Ca2+ indicator by systematically exploring the relative configuration of dye and chelator, which can be targeted using the HaloTag self-labeling tag system. Our "isomeric tuning" approach is generalizable, yielding a farred targetable indicator to visualize Ca2+ fluxes in the primary cilium.