N-(6-甲基-2-苯并噻唑基)-2-[(3,4,6,7-四氢-3-(2-甲氧基苯基)-4-氧噻吩并[3,2-d]嘧啶-2-基)硫代]-乙酰胺 | 686772-17-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 噻吩并嘧啶酮衍生物
• 中文名称: N-(6-甲基-2-苯并噻唑基)-2-[(3,4,6,7-四氢-3-(2-甲氧基苯基)-4-氧噻吩并[3,2-d]嘧啶-2-基)硫代]-乙酰胺
• 英文名称: 2-((3-(2-methoxyphenyl)-4-oxo-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide
• 英文别名: 2-[[3-(2-methoxyphenyl)-4-oxo-6,7-dihydrothieno[3,2-d]pyrimidin-2-yl]sulfanyl]-N-(6-methyl-1,3-benzothiazol-2-yl)acetamide
• CAS: 686772-17-8
• 化学式: C₂₃H₂₀N₄O₃S₃
• 分子量: 496.6
• InChiKey: RHUJMHOIQBDFQR-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.8
• 重原子数：
  33
• 可旋转键数：
  6
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  163
• 氢给体数：
  1
• 氢受体数：
  8

文献信息

• CELL AGGREGATION INCLUDING OLFACTORY NEURON OR PRECURSOR CELL THEREOF, AND METHOD FOR PRODUCING SAME
  申请人：SUMITOMO CHEMICAL COMPANY LIMITED

  公开号：EP3842527A1

  公开（公告）日：2021-06-30

  A method for producing a cell cluster including an olfactory receptor neuron or a precursor cell thereof, comprising the following steps (1) to (3): step (1) of suspension-culturing a pluripotent stem cell in the presence of a Wnt signaling pathway inhibitory substance to form a cell aggregate; step (2) of suspension-culturing the cell aggregate obtained in the step (1) in the presence of a BMP signaling pathway-activating substance; and step (3) of suspension-culturing the cell aggregate obtained in the step (2) to obtain the cell cluster, wherein step (3) comprises at least one step selected from the group consisting of: step (3a) of suspension-culturing in the presence of an FGF signaling pathway-activating substance; step (3b) of suspension-culturing in the presence of a BMP signaling pathway inhibitory substance; and step (3c) of culturing in the presence of an FGF signaling pathway-activating substance and a BMP signaling pathway inhibitory substance.

  一种生产包括嗅觉受体神经元或其前体细胞的细胞团的方法，包括以下步骤(1)至(3)：步骤(1)在Wnt信号通路抑制物质存在下悬浮培养多能干细胞以形成细胞聚集体；步骤(2)在BMP信号通路激活物质存在下悬浮培养步骤(1)中获得的细胞聚集体；以及步骤(3)悬浮培养步骤(2)中获得的细胞聚集体以获得细胞簇，其中步骤(3)包括至少一个选自由以下组成的组的步骤：在 FGF 信号通路激活物质存在下进行悬浮培养的步骤(3a)； 在 BMP 信号通路抑制物质存在下进行悬浮培养的步骤(3b)；以及 在 FGF 信号通路激活物质和 BMP 信号通路抑制物质存在下进行培养的步骤(3c)。
• Methods for epicardial differentiation of human pluripotent stem cells
  申请人：Wisconsin Alumni Research Foundation

  公开号：US10131878B2

  公开（公告）日：2018-11-20

  Methods for generating high-yield, high-purity epicardial cells are described. Wnt/β-catenin signaling is first activated in human cardiac progenitor cells, by, for example, inhibiting Gsk-3 to induce differentiation into epicardial cells. Methods for long-term in vitro maintenance of human cardiac progenitor cell-derived epicardial cells and method comprising chemically defined, xeno-free, and albumin-free culture conditions are also provided.

  本文介绍了产生高产、高纯度心外膜细胞的方法。Wnt/β-catenin 信号首先在人类心脏祖细胞中激活，例如通过抑制 Gsk-3 来诱导分化为心外膜细胞。此外，还提供了长期体外维持人心脏祖细胞衍生的心外膜细胞的方法，以及包括化学定义、无异种和无白蛋白培养条件的方法。
• MACS-based purification of stem cell-derived retinal pigment epithelium
  申请人：FUJIFILM Cellular Dynamics, Inc.

  公开号：US11162070B2

  公开（公告）日：2021-11-02

  Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and/or CD90 positive cells from a starting population of RPE cells.

  本文提供了富集源自干细胞的视网膜色素上皮（RPE）细胞群的方法。这种方法可包括从RPE细胞起始群体中去除CD24阳性细胞、CD56阳性细胞和/或CD90阳性细胞，从而去除污染细胞。
• Method of making cardiomyocytes from human pluripotent cells
  申请人：Wisconsin Alumni Research Foundation

  公开号：US11352604B2

  公开（公告）日：2022-06-07

  An improvement to the GiWi protocol for differentiating human pluripotent cells to developmentally mature cardiomyocytes includes a step of activating innate immunity in mesoderm stage cells in the in vitro differentiation culture. When the mesoderm cells, which are precursors to cardiac progenitor cells, are primed by exposure to an activator of innate immunity, a population of cardiomyocytes is generated that is more developmentally mature than is generated in the GiWi protocol without the primed step. Also provided herein are in vitro ventricular conductive microtissues and isolated, in vitro populations of ventricular conduction system-like cells and methods for making the same.

  将人类多能细胞分化为发育成熟的心肌细胞的 GiWi 方案的改进包括在体外分化培养中激活中胚层细胞的先天性免疫。中胚层细胞是心脏祖细胞的前体，当它们暴露于先天性免疫激活剂中时，生成的心肌细胞群比没有激活步骤的 GiWi 方案中生成的心肌细胞群发育得更成熟。本文还提供了体外心室传导微组织和分离的体外心室传导系统样细胞群，以及制造这些微组织和细胞的方法。
• METHOD, COMBINATION AND/OR COMPOSITION FOR INDUCING CARDIOMYOCYTE DIFFERENTATION
  申请人：Singapore Health Services Pte Ltd

  公开号：EP2882848A1

  公开（公告）日：2015-06-17